Single-cell Analysis of Lambda Immunity Regulation
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Single-cell Analysis of Lambda Immunity Regulation. / Bæk, Kristoffer Torbjørn; Svenningsen, Sine Lo; Eisen, Harvey; Sneppen, Kim; Brown, Stanley.
I: Journal of Molecular Biology, Bind 334, Nr. 3, 2003, s. 363-372.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Single-cell Analysis of Lambda Immunity Regulation
AU - Bæk, Kristoffer Torbjørn
AU - Svenningsen, Sine Lo
AU - Eisen, Harvey
AU - Sneppen, Kim
AU - Brown, Stanley
N1 - Keywords: bacteriophage lambda; repressor; GFP; single-cell analyses
PY - 2003
Y1 - 2003
N2 - We have examined expression of the ¿cI operon in single cells via a rexgfp substitution. Although average fluorescence agreed with expectations for expression of ¿-repressor, fluorescence fluctuated greatly from cell-to-cell. Fluctuations in repressor concentration are not predicted by previous models and are tolerated in part by a regulatory response to DNA damage.
AB - We have examined expression of the ¿cI operon in single cells via a rexgfp substitution. Although average fluorescence agreed with expectations for expression of ¿-repressor, fluorescence fluctuated greatly from cell-to-cell. Fluctuations in repressor concentration are not predicted by previous models and are tolerated in part by a regulatory response to DNA damage.
U2 - 10.1016/j.jmb.2003.09.037
DO - 10.1016/j.jmb.2003.09.037
M3 - Journal article
VL - 334
SP - 363
EP - 372
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
SN - 0022-2836
IS - 3
ER -
ID: 17582760