Towards a standardized bioinformatics infrastructure for N- and O-glycomics

Research output: Contribution to journalReviewResearchpeer-review


  • Miguel A. Rojas-Macias
  • Julien Mariethoz
  • Peter Andersson
  • Chunsheng Jin
  • Vignesh Venkatakrishnan
  • Nobuyuki P. Aoki
  • Daisuke Shinmachi
  • Christopher Ashwood
  • Katarina Madunic
  • Tao Zhang
  • Oliver Horlacher
  • Weston B. Struwe
  • Yu Watanabe
  • Shujiro Okuda
  • Fredrik Levander
  • Daniel Kolarich
  • Pauline M. Rudd
  • Manfred Wuhrer
  • Carsten Kettner
  • Nicolle H. Packer
  • Kiyoko F. Aoki-Kinoshita
  • Frédérique Lisacek
  • Niclas G. Karlsson

The mass spectrometry (MS)-based analysis of free polysaccharides and glycans released from proteins, lipids and proteoglycans increasingly relies on databases and software. Here, we review progress in the bioinformatics analysis of protein-released N- and O-linked glycans (N- and O-glycomics) and propose an e-infrastructure to overcome current deficits in data and experimental transparency. This workflow enables the standardized submission of MS-based glycomics information into the public repository UniCarb-DR. It implements the MIRAGE (Minimum Requirement for A Glycomics Experiment) reporting guidelines, storage of unprocessed MS data in the GlycoPOST repository and glycan structure registration using the GlyTouCan registry, thereby supporting the development and extension of a glycan structure knowledgebase.

Original languageEnglish
Article number3275
JournalNature Communications
Issue number1
Number of pages10
Publication statusPublished - 2019

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