The AMSH3 ESCRT-III-Associated Deubiquitinase Is Essential for Plant Immunity
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The AMSH3 ESCRT-III-Associated Deubiquitinase Is Essential for Plant Immunity. / Schultz-Larsen, Torsten; Lenk, Andrea; Kalinowska, Kamila; Vestergaard, Lau Kræsing; Pedersen, Carsten; Isono, Erika; Thordal-Christensen, Hans.
In: Cell Reports, Vol. 25, No. 9, 2018, p. 2329-2338.e5.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The AMSH3 ESCRT-III-Associated Deubiquitinase Is Essential for Plant Immunity
AU - Schultz-Larsen, Torsten
AU - Lenk, Andrea
AU - Kalinowska, Kamila
AU - Vestergaard, Lau Kræsing
AU - Pedersen, Carsten
AU - Isono, Erika
AU - Thordal-Christensen, Hans
PY - 2018
Y1 - 2018
N2 - Plant “nucleotide-binding leucine-rich repeat” receptor proteins (NLRs) detect alterations in host targets of pathogen effectors and trigger immune responses. The Arabidopsis thaliana mutant pen1 syp122 displays autoimmunity, and a mutant screen identified the deubiquitinase “associated molecule with the SH3 domain of STAM3” (AMSH3) to be required for this phenotype. AMSH3 has previously been implicated in ESCRT-mediated vacuolar targeting. Pathology experiments show that AMSH3 activity is required for immunity mediated by the CC-NLRs, RPS2 and RPM1. Co-expressing the autoactive RPM1 D505V and the catalytically inactive ESCRT-III protein SKD1 E232Q in Nicotiana benthamiana supports the requirement of ESCRT-associated functions for this CC-NLR-activated immunity. Meanwhile, loss of ESCRT function in A. thaliana is lethal, and we find that AMSH3 knockout-triggered seedling lethality is “enhanced disease susceptibility 1” (EDS1) dependent. Future studies may reveal whether AMSH3 is monitored by a TIR-NLR immunity receptor. NLR receptors are important for plant immunity as they monitor plant processes that are targeted by pathogens. Schultz-Larsen et al. find that the AMSH3 deubiquitinase enzyme is important for immunity activated by specific NLRs. Furthermore, their results suggest that AMSH3 itself is monitored by other NLRs.
AB - Plant “nucleotide-binding leucine-rich repeat” receptor proteins (NLRs) detect alterations in host targets of pathogen effectors and trigger immune responses. The Arabidopsis thaliana mutant pen1 syp122 displays autoimmunity, and a mutant screen identified the deubiquitinase “associated molecule with the SH3 domain of STAM3” (AMSH3) to be required for this phenotype. AMSH3 has previously been implicated in ESCRT-mediated vacuolar targeting. Pathology experiments show that AMSH3 activity is required for immunity mediated by the CC-NLRs, RPS2 and RPM1. Co-expressing the autoactive RPM1 D505V and the catalytically inactive ESCRT-III protein SKD1 E232Q in Nicotiana benthamiana supports the requirement of ESCRT-associated functions for this CC-NLR-activated immunity. Meanwhile, loss of ESCRT function in A. thaliana is lethal, and we find that AMSH3 knockout-triggered seedling lethality is “enhanced disease susceptibility 1” (EDS1) dependent. Future studies may reveal whether AMSH3 is monitored by a TIR-NLR immunity receptor. NLR receptors are important for plant immunity as they monitor plant processes that are targeted by pathogens. Schultz-Larsen et al. find that the AMSH3 deubiquitinase enzyme is important for immunity activated by specific NLRs. Furthermore, their results suggest that AMSH3 itself is monitored by other NLRs.
KW - AMSH3 deubiquitinase
KW - ESCRT
KW - autoimmunity
KW - nucleotide-binding leucine-rich repeat receptors
KW - plant immunity
U2 - 10.1016/j.celrep.2018.11.011
DO - 10.1016/j.celrep.2018.11.011
M3 - Journal article
C2 - 30485803
VL - 25
SP - 2329-2338.e5
JO - Cell Reports
JF - Cell Reports
SN - 2211-1247
IS - 9
ER -
ID: 209806323