Swelling lecithin: cholesterol implants for the controlled release of proteins
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Swelling lecithin : cholesterol implants for the controlled release of proteins. / Tantipolphan, Ruedeeporn; Rades, Thomas; Medlicott, Natalie J.
In: Journal of Liposome Research, Vol. 19, No. 1, 2009, p. 37-48.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Swelling lecithin
T2 - cholesterol implants for the controlled release of proteins
AU - Tantipolphan, Ruedeeporn
AU - Rades, Thomas
AU - Medlicott, Natalie J
PY - 2009
Y1 - 2009
N2 - This work demonstrated the effect of two salts as potential simple formulation excipients in modifying hydration properties, phase behavior, and protein release from lecithin-based implants. In vitro release of a model protein, bovine serum albumin (BSA), from cylindrical-shaped lecithin and lecithin:cholesterol (1:1 w/w) implants containing 0, 10, or 30% w/w NaCl or CaCl2 was studied. In the absence of salts, BSA was released from lecithin and lecithin:cholesterol implants with a high monomer content and the release profiles were similar to those previously reported. Cholesterol increased the swelling, induced the formation of myelin structures, and reduced BSA release from the matrices. Addition of the salts to lecithin:cholesterol implants further enhanced the swelling, altered the hydrated morphology, and inhibited protein release. Analyses showed that BSA associated into multimers within these swollen lipid matrices but retained a high degree of protein native structure. Factors that may have contributed to the inhibition of the in vitro release included 1) the swollen multilamellar layers assembled as diffusional barriers, 2) adsorption of BSA onto the hydrated lipid vesicles, and 3) formation of protein aggregates.
AB - This work demonstrated the effect of two salts as potential simple formulation excipients in modifying hydration properties, phase behavior, and protein release from lecithin-based implants. In vitro release of a model protein, bovine serum albumin (BSA), from cylindrical-shaped lecithin and lecithin:cholesterol (1:1 w/w) implants containing 0, 10, or 30% w/w NaCl or CaCl2 was studied. In the absence of salts, BSA was released from lecithin and lecithin:cholesterol implants with a high monomer content and the release profiles were similar to those previously reported. Cholesterol increased the swelling, induced the formation of myelin structures, and reduced BSA release from the matrices. Addition of the salts to lecithin:cholesterol implants further enhanced the swelling, altered the hydrated morphology, and inhibited protein release. Analyses showed that BSA associated into multimers within these swollen lipid matrices but retained a high degree of protein native structure. Factors that may have contributed to the inhibition of the in vitro release included 1) the swollen multilamellar layers assembled as diffusional barriers, 2) adsorption of BSA onto the hydrated lipid vesicles, and 3) formation of protein aggregates.
U2 - 10.1080/08982100802636434
DO - 10.1080/08982100802636434
M3 - Journal article
C2 - 19515006
VL - 19
SP - 37
EP - 48
JO - Journal of Liposome Research
JF - Journal of Liposome Research
SN - 0898-2104
IS - 1
ER -
ID: 40349212