Lactate in cystic fibrosis sputum
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Lactate in cystic fibrosis sputum. / Bensel, Tobias; Stotz, Martin; Borneff-Lipp, Marianne; Wollschläger, Bettina; Wienke, Andreas; Taccetti, Giovanni; Campana, Silvia; Meyer, Keith C; Jensen, Peter Østrup; Lechner, Ute; Ulrich, Martina; Döring, Gerd; Worlitzsch, Dieter.
In: Journal of Cystic Fibrosis, Vol. 10, No. 1, 2011, p. 37-44.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Lactate in cystic fibrosis sputum
AU - Bensel, Tobias
AU - Stotz, Martin
AU - Borneff-Lipp, Marianne
AU - Wollschläger, Bettina
AU - Wienke, Andreas
AU - Taccetti, Giovanni
AU - Campana, Silvia
AU - Meyer, Keith C
AU - Jensen, Peter Østrup
AU - Lechner, Ute
AU - Ulrich, Martina
AU - Döring, Gerd
AU - Worlitzsch, Dieter
N1 - Copyright © 2010 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.
PY - 2011
Y1 - 2011
N2 - Antibiotic therapy is thought to improve lung function in patients with cystic fibrosis (CF) by decreasing neutrophil-derived inflammation. We investigated the origin and clinical significance of lactate in the chronically inflamed CF lung. Methods Lactate was measured in sputa of 18 exacerbated and 25 stable CF patients via spectrophotometry and gaschromatography. Lung function was assessed via spirometry. Seven patients with chronic obstructive pulmonary disease (COPD) and three patients with acute lung inflammation served as control groups. Neutrophil and bacterial lactate production was assessed under aerobic and anaerobic conditions. Results In sputum specimens of patients with respiratory exacerbations lactate concentrations decreased significantly (p < 0.005) from 3.4 ± 2.3 mmol/L to 1.4 ± 1.4 mmol/L after 2–3 weeks of intravenous antibiotics. Successful treatment was reflected in 16 patients (88.9%) by FVC increase associated with lactate decrease (p < 0.05). In every single sputum lactate was detectable (3.0 ± 3.1 mmol/L, range 0.2–14.1 mmol/L). Lactate was lower (1.6 ± 0.8 mmol/L) in sputa from seven COPD patients, and it was below the detection limit in three patients with acute lung inflammation. Neutrophil lactate production accumulated up to 10.5 mmol/L after 4 days, whereas bacterial lactate production did not appear to contribute substantially to sputum lactate concentrations. Conclusions Successful antibiotic therapy is reflected by a decrease in lactate concentrations. Neutrophils are the most likely source for lactate in sputum of CF patients. Therefore lactate may be used to monitor responses to antibiotic therapy as an adjunct to lung function measurements.
AB - Antibiotic therapy is thought to improve lung function in patients with cystic fibrosis (CF) by decreasing neutrophil-derived inflammation. We investigated the origin and clinical significance of lactate in the chronically inflamed CF lung. Methods Lactate was measured in sputa of 18 exacerbated and 25 stable CF patients via spectrophotometry and gaschromatography. Lung function was assessed via spirometry. Seven patients with chronic obstructive pulmonary disease (COPD) and three patients with acute lung inflammation served as control groups. Neutrophil and bacterial lactate production was assessed under aerobic and anaerobic conditions. Results In sputum specimens of patients with respiratory exacerbations lactate concentrations decreased significantly (p < 0.005) from 3.4 ± 2.3 mmol/L to 1.4 ± 1.4 mmol/L after 2–3 weeks of intravenous antibiotics. Successful treatment was reflected in 16 patients (88.9%) by FVC increase associated with lactate decrease (p < 0.05). In every single sputum lactate was detectable (3.0 ± 3.1 mmol/L, range 0.2–14.1 mmol/L). Lactate was lower (1.6 ± 0.8 mmol/L) in sputa from seven COPD patients, and it was below the detection limit in three patients with acute lung inflammation. Neutrophil lactate production accumulated up to 10.5 mmol/L after 4 days, whereas bacterial lactate production did not appear to contribute substantially to sputum lactate concentrations. Conclusions Successful antibiotic therapy is reflected by a decrease in lactate concentrations. Neutrophils are the most likely source for lactate in sputum of CF patients. Therefore lactate may be used to monitor responses to antibiotic therapy as an adjunct to lung function measurements.
KW - Adolescent
KW - Adult
KW - Aged
KW - Anti-Bacterial Agents
KW - Bacteria
KW - Child
KW - Chromatography, Gas
KW - Comparative Effectiveness Research
KW - Cystic Fibrosis
KW - Drug Monitoring
KW - Female
KW - Humans
KW - Lactic Acid
KW - Male
KW - Middle Aged
KW - Neutrophils
KW - Pneumonia
KW - Respiratory Function Tests
KW - Spectrophotometry
KW - Sputum
KW - Treatment Outcome
U2 - 10.1016/j.jcf.2010.09.004
DO - 10.1016/j.jcf.2010.09.004
M3 - Journal article
C2 - 20947455
VL - 10
SP - 37
EP - 44
JO - Journal of Cystic Fibrosis
JF - Journal of Cystic Fibrosis
SN - 1569-1993
IS - 1
ER -
ID: 38517894