IAP-Based Cell Sorting Results in Homogeneous Transplantable Dopaminergic Precursor Cells Derived from Human Pluripotent Stem Cells.

Research output: Contribution to journalJournal articleResearchpeer-review


  • Daniela Lehnen
  • Serena Barral
  • Tiago Cardoso
  • Shane Grealish
  • Andreas Heuer
  • Andrej Smiyakin
  • Kirkeby, Agnete
  • Jutta Kollet
  • Harold Cremer
  • Malin Parmar
  • Andreas Bosio
  • Sebastian. Knoebel
Human pluripotent stem cell (hPSC)-derived mesencephalic dopaminergic (mesDA) neurons can relieve motor deficits in animal models of Parkinson's disease (PD). Clin. translation of differentiation protocols requires standardization of prodn. procedures, and surface-marker-based cell sorting is considered instrumental for reproducible generation of defined cell products. Here, we demonstrate that integrin-assocd. protein (IAP) is a cell surface marker suitable for enrichment of hPSC-derived mesDA progenitor cells. Immunomagnetically sorted IAP+ mesDA progenitors showed increased expression of ventral midbrain floor plate markers, lacked expression of pluripotency markers, and differentiated into mature dopaminergic (DA) neurons in vitro. Intrastriatal transplantation of IAP+ cells sorted at day 16 of differentiation in a rat model of PD resulted in functional recovery. Grafts from sorted IAP+ mesDA progenitors were more homogeneous in size and DA neuron d. Thus, we suggest IAP-based sorting for reproducible prospective enrichment of mesDA progenitor cells in clin. cell replacement strategies. [on SciFinder(R)]
Original languageEnglish
JournalStem Cell Reports
Issue number4
Pages (from-to)1207-1220
Number of pages14
Publication statusPublished - Oct 2017

Bibliographical note

M1 - Copyright (C) 2018 American Chemical Society (ACS). All Rights Reserved.

CAPLUS AN 2017:1573803(Journal; Online Computer File)

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