Human skeletal muscle ceramide content is not a major factor in muscle insulin sensitivity

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AIMS/HYPOTHESIS: In skeletal muscle, ceramides may be involved in the pathogenesis of insulin resistance through an attenuation of insulin signalling. This study investigated total skeletal muscle ceramide fatty acid content in participants exhibiting a wide range of insulin sensitivities. METHODS: The middle-aged male participants (n=33) were matched for lean body mass and divided into four groups: type 2 diabetes (T2D, n=8), impaired glucose tolerance (IGT, n=9), healthy controls (CON, n=8) and endurance-trained (TR, n=8). A two step (28 and 80 mU m(-2) min(-1)) sequential euglycaemic-hyperinsulinaemic clamp was performed for 120 and 90 min for step 1 and step 2, respectively. Muscle biopsies were obtained from vastus lateralis at baseline, and after steps 1 and 2. RESULTS: Glucose infusion rates increased in response to insulin infusion, and significant differences were present between groups (T2D
Original languageEnglish
Issue number7
Pages (from-to)1253-60
Number of pages7
Publication statusPublished - 2008

Bibliographical note

Keywords: Biopsy; Blood Glucose; Ceramides; Diabetes Mellitus, Type 2; Diglycerides; Glucose Clamp Technique; Glucose Intolerance; Humans; Hyperinsulinism; Insulin Resistance; Male; Middle Aged; Muscle, Skeletal; Oxygen Consumption; Physical Endurance; Triglycerides

ID: 10118890