Formulation optimization, anesthetic activity, skin permeation, and transportation pathway of Alpinia galanga oil SNEDDS in zebrafish (Danio rerio)

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Formulation optimization, anesthetic activity, skin permeation, and transportation pathway of Alpinia galanga oil SNEDDS in zebrafish (Danio rerio). / Khumpirapang, Nattakanwadee; von Gersdorff Jørgensen, Louise; Müllertz, Anette; Rades, Thomas; Okonogi, Siriporn.

In: European Journal of Pharmaceutics and Biopharmaceutics, Vol. 165, 2021, p. 193-202.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Khumpirapang, N, von Gersdorff Jørgensen, L, Müllertz, A, Rades, T & Okonogi, S 2021, 'Formulation optimization, anesthetic activity, skin permeation, and transportation pathway of Alpinia galanga oil SNEDDS in zebrafish (Danio rerio)', European Journal of Pharmaceutics and Biopharmaceutics, vol. 165, pp. 193-202. https://doi.org/10.1016/j.ejpb.2021.04.022

APA

Khumpirapang, N., von Gersdorff Jørgensen, L., Müllertz, A., Rades, T., & Okonogi, S. (2021). Formulation optimization, anesthetic activity, skin permeation, and transportation pathway of Alpinia galanga oil SNEDDS in zebrafish (Danio rerio). European Journal of Pharmaceutics and Biopharmaceutics, 165, 193-202. https://doi.org/10.1016/j.ejpb.2021.04.022

Vancouver

Khumpirapang N, von Gersdorff Jørgensen L, Müllertz A, Rades T, Okonogi S. Formulation optimization, anesthetic activity, skin permeation, and transportation pathway of Alpinia galanga oil SNEDDS in zebrafish (Danio rerio). European Journal of Pharmaceutics and Biopharmaceutics. 2021;165:193-202. https://doi.org/10.1016/j.ejpb.2021.04.022

Author

Khumpirapang, Nattakanwadee ; von Gersdorff Jørgensen, Louise ; Müllertz, Anette ; Rades, Thomas ; Okonogi, Siriporn. / Formulation optimization, anesthetic activity, skin permeation, and transportation pathway of Alpinia galanga oil SNEDDS in zebrafish (Danio rerio). In: European Journal of Pharmaceutics and Biopharmaceutics. 2021 ; Vol. 165. pp. 193-202.

Bibtex

@article{80020ff532984c4fbfc5a65c696f524f,

title = "Formulation optimization, anesthetic activity, skin permeation, and transportation pathway of Alpinia galanga oil SNEDDS in zebrafish (Danio rerio)",

abstract = "Alpinia galanga oil (AGO) has an anesthetic activity but its water insoluble property limits its clinical applications. The aim of the present study was to develop a self-nanoemulsifying drug delivery system of AGO (SNEDDS-AGO) to avoid the use of organic solvent and investigate AGO transportation pathway and anesthetic activity. Three optimized formulations from a contour plots of droplet size; SNEDDS-AGO-1, SNEDDS-AGO-2, and SNEDDS-AGO-3, composed of AGO, Miglyol 812, Cremophor RH 40, Capmul MCM EP, and ethanol at the ratios of 40:10:35:10:5, 40:20:15:20:5, and 60:10:15:10:5, respectively were selected as they possessed different droplet size of 62 ± 0.5, 107 ± 2.8, and 207 ± 4.3 nm, respectively. It was found that the droplet size played an important role in fish anesthesia. SNEDDS-AGO-3 showed the longest anesthetic induction time (270 sec) (p < 0.03). Transportation pathway and skin permeation of SNEDDS-AGO-2 were investigated using nile red labelled AGO and detected by fluorescence microscope. AGO was found mostly in brain, gills, and skin suggesting that the transportation pathway of AGO in zebrafish is passing through the gills and skin to the brain. SNEDDS-AGO formulations showed significantly higher permeation through the skin than AGO ethanolic solution. In conclusion, SNEDDS is a promising delivery system of AGO.",

keywords = "Alpinia galanga, Anesthetic effect, Drug delivery system, Skin permeation, SNEDDS, Transportation pathway, Zebrafish (Danio rerio)",

author = "Nattakanwadee Khumpirapang and {von Gersdorff J{\o}rgensen}, Louise and Anette M{\"u}llertz and Thomas Rades and Siriporn Okonogi",

note = "Publisher Copyright: {\textcopyright} 2021 Elsevier B.V.",

year = "2021",

doi = "10.1016/j.ejpb.2021.04.022",

language = "English",

volume = "165",

pages = "193--202",

journal = "European Journal of Pharmaceutics and Biopharmaceutics",

issn = "0939-6411",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Formulation optimization, anesthetic activity, skin permeation, and transportation pathway of Alpinia galanga oil SNEDDS in zebrafish (Danio rerio)

AU - Khumpirapang, Nattakanwadee

AU - von Gersdorff Jørgensen, Louise

AU - Müllertz, Anette

AU - Rades, Thomas

AU - Okonogi, Siriporn

PY - 2021

Y1 - 2021

N2 - Alpinia galanga oil (AGO) has an anesthetic activity but its water insoluble property limits its clinical applications. The aim of the present study was to develop a self-nanoemulsifying drug delivery system of AGO (SNEDDS-AGO) to avoid the use of organic solvent and investigate AGO transportation pathway and anesthetic activity. Three optimized formulations from a contour plots of droplet size; SNEDDS-AGO-1, SNEDDS-AGO-2, and SNEDDS-AGO-3, composed of AGO, Miglyol 812, Cremophor RH 40, Capmul MCM EP, and ethanol at the ratios of 40:10:35:10:5, 40:20:15:20:5, and 60:10:15:10:5, respectively were selected as they possessed different droplet size of 62 ± 0.5, 107 ± 2.8, and 207 ± 4.3 nm, respectively. It was found that the droplet size played an important role in fish anesthesia. SNEDDS-AGO-3 showed the longest anesthetic induction time (270 sec) (p < 0.03). Transportation pathway and skin permeation of SNEDDS-AGO-2 were investigated using nile red labelled AGO and detected by fluorescence microscope. AGO was found mostly in brain, gills, and skin suggesting that the transportation pathway of AGO in zebrafish is passing through the gills and skin to the brain. SNEDDS-AGO formulations showed significantly higher permeation through the skin than AGO ethanolic solution. In conclusion, SNEDDS is a promising delivery system of AGO.

AB - Alpinia galanga oil (AGO) has an anesthetic activity but its water insoluble property limits its clinical applications. The aim of the present study was to develop a self-nanoemulsifying drug delivery system of AGO (SNEDDS-AGO) to avoid the use of organic solvent and investigate AGO transportation pathway and anesthetic activity. Three optimized formulations from a contour plots of droplet size; SNEDDS-AGO-1, SNEDDS-AGO-2, and SNEDDS-AGO-3, composed of AGO, Miglyol 812, Cremophor RH 40, Capmul MCM EP, and ethanol at the ratios of 40:10:35:10:5, 40:20:15:20:5, and 60:10:15:10:5, respectively were selected as they possessed different droplet size of 62 ± 0.5, 107 ± 2.8, and 207 ± 4.3 nm, respectively. It was found that the droplet size played an important role in fish anesthesia. SNEDDS-AGO-3 showed the longest anesthetic induction time (270 sec) (p < 0.03). Transportation pathway and skin permeation of SNEDDS-AGO-2 were investigated using nile red labelled AGO and detected by fluorescence microscope. AGO was found mostly in brain, gills, and skin suggesting that the transportation pathway of AGO in zebrafish is passing through the gills and skin to the brain. SNEDDS-AGO formulations showed significantly higher permeation through the skin than AGO ethanolic solution. In conclusion, SNEDDS is a promising delivery system of AGO.

KW - Alpinia galanga

KW - Anesthetic effect

KW - Drug delivery system

KW - Skin permeation

KW - SNEDDS

KW - Transportation pathway

KW - Zebrafish (Danio rerio)

U2 - 10.1016/j.ejpb.2021.04.022

DO - 10.1016/j.ejpb.2021.04.022

M3 - Journal article

C2 - 33979660

AN - SCOPUS:85107018141

VL - 165

SP - 193

EP - 202

JO - European Journal of Pharmaceutics and Biopharmaceutics

JF - European Journal of Pharmaceutics and Biopharmaceutics

SN - 0939-6411

ER -

ID: 272069651