Endo- and exocytic rate constants for spontaneous and protein kinase C-activated T cell receptor cycling
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Endo- and exocytic rate constants for spontaneous and protein kinase C-activated T cell receptor cycling. / Menné, Charlotte; Møller Sørensen, Tine; Siersma, Volkert; von Essen, Marina; Ødum, Niels; Geisler, Carsten.
In: European Journal of Immunology, Vol. 32, No. 3, 2002, p. 616-26.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Endo- and exocytic rate constants for spontaneous and protein kinase C-activated T cell receptor cycling
AU - Menné, Charlotte
AU - Møller Sørensen, Tine
AU - Siersma, Volkert
AU - von Essen, Marina
AU - Ødum, Niels
AU - Geisler, Carsten
N1 - Keywords: Amino Acid Motifs; Antibodies, Monoclonal; Antigens, CD3; Down-Regulation; Endocytosis; Enzyme Activation; Exocytosis; Fluorescein-5-isothiocyanate; Fluorescent Dyes; Humans; Jurkat Cells; Kinetics; Phosphorylation; Protein Kinase C; Protein Processing, Post-Translational; Receptors, Antigen, T-Cell; Up-Regulation
PY - 2002
Y1 - 2002
N2 - To determine the rate constants of spontaneous and activated TCR cycling, we examined TCR endo- and exocytosis in the human T cell line Jurkat by three different methods. Using a simple kinetic model for TCR cycling and non-linear regression analyses, we found that the spontaneous endocytic rate constant of the TCR was low (approximately 0.012 min(-1)) whereas the spontaneous exocytic rate constant was similar to that of other cycling receptors (approximately 0.055 min(-1)). Following protein kinase C activation (PKC) the endocytic rate constant was increased tenfold (to approximately 0.128 min(-1)) whereas the exocytic rate constant was unaffected. Thus, the TCR becomes a rapidly cycling receptor with kinetics similar to classical cycling receptors subsequent to PKC activation. This results in a reduction of the half-life of cell surface expressed TCR from approximately 58 to 6 min and allows rapid redistribution of the TCR during T cell activation.
AB - To determine the rate constants of spontaneous and activated TCR cycling, we examined TCR endo- and exocytosis in the human T cell line Jurkat by three different methods. Using a simple kinetic model for TCR cycling and non-linear regression analyses, we found that the spontaneous endocytic rate constant of the TCR was low (approximately 0.012 min(-1)) whereas the spontaneous exocytic rate constant was similar to that of other cycling receptors (approximately 0.055 min(-1)). Following protein kinase C activation (PKC) the endocytic rate constant was increased tenfold (to approximately 0.128 min(-1)) whereas the exocytic rate constant was unaffected. Thus, the TCR becomes a rapidly cycling receptor with kinetics similar to classical cycling receptors subsequent to PKC activation. This results in a reduction of the half-life of cell surface expressed TCR from approximately 58 to 6 min and allows rapid redistribution of the TCR during T cell activation.
M3 - Journal article
C2 - 11857335
VL - 32
SP - 616
EP - 626
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 3
ER -
ID: 8544638