Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain

Research output: Contribution to journal › Journal article › peer-review

Standard

Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain. / Brown, Jenny M.; Phan, Bao Anh; Aalling, Nadia; Morton, Gregory J.; Schwartz, Michael W.; Scarlett, Jarrad M.

In: STAR Protocols, Vol. 3, No. 2, 101329, 2022.

Research output: Contribution to journal › Journal article › peer-review

Harvard

Brown, JM, Phan, BA, Aalling, N, Morton, GJ, Schwartz, MW & Scarlett, JM 2022, 'Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain', STAR Protocols, vol. 3, no. 2, 101329. https://doi.org/10.1016/j.xpro.2022.101329

APA

Brown, J. M., Phan, B. A., Aalling, N., Morton, G. J., Schwartz, M. W., & Scarlett, J. M. (2022). Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain. STAR Protocols, 3(2), [101329]. https://doi.org/10.1016/j.xpro.2022.101329

Vancouver

Brown JM, Phan BA, Aalling N, Morton GJ, Schwartz MW, Scarlett JM. Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain. STAR Protocols. 2022;3(2). 101329. https://doi.org/10.1016/j.xpro.2022.101329

Author

Brown, Jenny M. ; Phan, Bao Anh ; Aalling, Nadia ; Morton, Gregory J. ; Schwartz, Michael W. ; Scarlett, Jarrad M. / Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain. In: STAR Protocols. 2022 ; Vol. 3, No. 2.

Bibtex

@article{02496d2ec8b84f328460058c71e96b20,
title = "Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain",
abstract = "Intracerebroventricular (icv) injection of fibroblast growth factor 1 (FGF1) elicits remission of diabetic hyperglycemia in rodent models of type 2 diabetes. Here, we present an optimized protocol to study the intracellular signaling pathways underlying the FGF1-induced sustained glucose lowering in the mouse brain. This protocol combines icv injection of FGF1 and osmotic mini-pump infusion of U0126, an inhibitor of MAPK/ERK signaling. We describe the surgical procedure and verification of U0126 inhibition of FGF1-stimulated hypothalamic MAPK/ERK signaling via western blot. For complete details on the use and execution of this protocol, please refer to Brown et al. (2021).",
keywords = "Metabolism, Model Organisms, Molecular Biology, Neuroscience, Signal Transduction",
author = "Brown, {Jenny M.} and Phan, {Bao Anh} and Nadia Aalling and Morton, {Gregory J.} and Schwartz, {Michael W.} and Scarlett, {Jarrad M.}",
note = "Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
doi = "10.1016/j.xpro.2022.101329",
language = "English",
volume = "3",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",

}

RIS

TY - JOUR

T1 - Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain

AU - Brown, Jenny M.

AU - Phan, Bao Anh

AU - Aalling, Nadia

AU - Morton, Gregory J.

AU - Schwartz, Michael W.

AU - Scarlett, Jarrad M.

N1 - Publisher Copyright: © 2022 The Author(s)

PY - 2022

Y1 - 2022

N2 - Intracerebroventricular (icv) injection of fibroblast growth factor 1 (FGF1) elicits remission of diabetic hyperglycemia in rodent models of type 2 diabetes. Here, we present an optimized protocol to study the intracellular signaling pathways underlying the FGF1-induced sustained glucose lowering in the mouse brain. This protocol combines icv injection of FGF1 and osmotic mini-pump infusion of U0126, an inhibitor of MAPK/ERK signaling. We describe the surgical procedure and verification of U0126 inhibition of FGF1-stimulated hypothalamic MAPK/ERK signaling via western blot. For complete details on the use and execution of this protocol, please refer to Brown et al. (2021).

AB - Intracerebroventricular (icv) injection of fibroblast growth factor 1 (FGF1) elicits remission of diabetic hyperglycemia in rodent models of type 2 diabetes. Here, we present an optimized protocol to study the intracellular signaling pathways underlying the FGF1-induced sustained glucose lowering in the mouse brain. This protocol combines icv injection of FGF1 and osmotic mini-pump infusion of U0126, an inhibitor of MAPK/ERK signaling. We describe the surgical procedure and verification of U0126 inhibition of FGF1-stimulated hypothalamic MAPK/ERK signaling via western blot. For complete details on the use and execution of this protocol, please refer to Brown et al. (2021).

KW - Metabolism

KW - Model Organisms

KW - Molecular Biology

KW - Neuroscience

KW - Signal Transduction

U2 - 10.1016/j.xpro.2022.101329

DO - 10.1016/j.xpro.2022.101329

M3 - Journal article

C2 - 35479117

AN - SCOPUS:85128379936

VL - 3

JO - STAR Protocols

JF - STAR Protocols

SN - 2666-1667

IS - 2

M1 - 101329

ER -

ID: 305684755