Choice of medium affects PBMC quantification, cell size, and downstream respiratory analysis

Research output: Working paper › Preprint › Research

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Choice of medium affects PBMC quantification, cell size, and downstream respiratory analysis. / Christensen, Ida Bager; Ribas, Lucas; Larsen, Steen; Dela, Flemming; Gillberg, Linn.

bioRxiv, 2022.

Research output: Working paper › Preprint › Research

Harvard

Christensen, IB, Ribas, L, Larsen, S, Dela, F & Gillberg, L 2022 'Choice of medium affects PBMC quantification, cell size, and downstream respiratory analysis' bioRxiv. https://doi.org/10.1101/2022.01.10.475633

APA

Christensen, I. B., Ribas, L., Larsen, S., Dela, F., & Gillberg, L. (2022). Choice of medium affects PBMC quantification, cell size, and downstream respiratory analysis. bioRxiv. https://doi.org/10.1101/2022.01.10.475633

Vancouver

Christensen IB, Ribas L, Larsen S, Dela F, Gillberg L. Choice of medium affects PBMC quantification, cell size, and downstream respiratory analysis. bioRxiv. 2022. https://doi.org/10.1101/2022.01.10.475633

Author

Christensen, Ida Bager ; Ribas, Lucas ; Larsen, Steen ; Dela, Flemming ; Gillberg, Linn. / Choice of medium affects PBMC quantification, cell size, and downstream respiratory analysis. bioRxiv, 2022.

Bibtex

@techreport{4ed9c6e39c034e4bb8d6418d1b5aaa77,
title = "Choice of medium affects PBMC quantification, cell size, and downstream respiratory analysis",
abstract = "High-resolution respirometry (HRR) can assess PBMC bioenergetics, but no standardized medium for PBMC preparation and HRR analysis exist. Here, we study the effect of four different media (MiR05, PBS, RPMI, Plasmax) on quantification, size, and HRR analysis (Oxygraph-O2k) of intact PBMCs. Remarkably, PBMC quantification was 21% higher in MiR05 than PBS and Plasmax, and 28% higher than in RPMI, causing O2 flux underestimation during HRR due to inherent adjustments. Moreover, smaller cell size of PBMCs and aggregation was observed in MiR05. We suggest optimization of HRR with a standardized, plasma-like medium for future HRR analysis of intact PBMCs.",
author = "Christensen, {Ida Bager} and Lucas Ribas and Steen Larsen and Flemming Dela and Linn Gillberg",
year = "2022",
doi = "10.1101/2022.01.10.475633",
language = "English",
volume = "2022",
publisher = "bioRxiv",
type = "WorkingPaper",
institution = "bioRxiv",

}

RIS

TY - UNPB

T1 - Choice of medium affects PBMC quantification, cell size, and downstream respiratory analysis

AU - Christensen, Ida Bager

AU - Ribas, Lucas

AU - Larsen, Steen

AU - Dela, Flemming

AU - Gillberg, Linn

PY - 2022

Y1 - 2022

N2 - High-resolution respirometry (HRR) can assess PBMC bioenergetics, but no standardized medium for PBMC preparation and HRR analysis exist. Here, we study the effect of four different media (MiR05, PBS, RPMI, Plasmax) on quantification, size, and HRR analysis (Oxygraph-O2k) of intact PBMCs. Remarkably, PBMC quantification was 21% higher in MiR05 than PBS and Plasmax, and 28% higher than in RPMI, causing O2 flux underestimation during HRR due to inherent adjustments. Moreover, smaller cell size of PBMCs and aggregation was observed in MiR05. We suggest optimization of HRR with a standardized, plasma-like medium for future HRR analysis of intact PBMCs.

AB - High-resolution respirometry (HRR) can assess PBMC bioenergetics, but no standardized medium for PBMC preparation and HRR analysis exist. Here, we study the effect of four different media (MiR05, PBS, RPMI, Plasmax) on quantification, size, and HRR analysis (Oxygraph-O2k) of intact PBMCs. Remarkably, PBMC quantification was 21% higher in MiR05 than PBS and Plasmax, and 28% higher than in RPMI, causing O2 flux underestimation during HRR due to inherent adjustments. Moreover, smaller cell size of PBMCs and aggregation was observed in MiR05. We suggest optimization of HRR with a standardized, plasma-like medium for future HRR analysis of intact PBMCs.

UR - https://doi.org/10.1101/2022.01.10.475633

U2 - 10.1101/2022.01.10.475633

DO - 10.1101/2022.01.10.475633

M3 - Preprint

VL - 2022

BT - Choice of medium affects PBMC quantification, cell size, and downstream respiratory analysis

PB - bioRxiv

ER -

ID: 333811989