Bovine cumulus-oocyte disconnection in vitro
Research output: Contribution to journal › Journal article › Research › peer-review
Cumulus-oocyte complexes were obtained from cows by aspiration of small (1-6 mm in diameter) antral follicles after slaughter. Complexes with a compact multilayered cumulus investment were cultured and processed for transmission electron microscopy after different periods of culture including a 0 h control group. In 0 h control oocytes the cumulus cells had numerous projections which penetrated the zona pellucida and established gap junctions with the oolemma. A partial loss of these junctions was noticed as an early event of oocyte maturation occurring within the first 3 h of culture. A low frequency of gap junctions was maintained until 12-18 h of culture where the junctional contact was completely disrupted. This decrease in intercellular communication was parallelled by resumption of oocyte meiosis.
|Journal||Brain Structure and Function (Print Edition)|
|Number of pages||4|
|Publication status||Published - 1987|
- Former LIFE faculty - Bovine, Oocyte maturation, Meiosis, Gap junction, Ultrastructure