Azone® decreases the buccal mucosal permeation of Diazepam in a concentration-dependent manner via a reservoir effect

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The purpose of this study was to examine concentration-dependent effects of Azone® (AZ) on the buccal absorption of diazepam (DIAZ). Porcine buccal mucosa was placed in modified Ussing chambers and pretreated with 10 μL of 0%, 5%, 20%, and 50% (w/v) AZ in ethanol. DIAZ was administered to the donor chamber either in solution or a chitosan-based gel. The donor chamber disappearance, receptor chamber appearance, and tissue retention of DIAZ were monitored over 2 h by HPLC, with AZ tissue disposition also measured by liquid chromatography-mass spectrometry profiling of tissue cryosections. DIAZ steady-state flux values significantly (p
Original languageEnglish
Article number103
JournalJournal of Pharmaceutical Sciences
Issue number4
Pages (from-to)1133-1141
Publication statusPublished - 15 Feb 2014

ID: 103025631