Acute and subacute pulmonary toxicity and mortality in mice after intratracheal instillation of ZnO nanoparticles in three laboratories

Research output: Contribution to journalJournal articlepeer-review

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Acute and subacute pulmonary toxicity and mortality in mice after intratracheal instillation of ZnO nanoparticles in three laboratories. / Jacobsen, Nicklas Raun; Stoeger, Tobias; van den Brule, Sybille; Saber, Anne Thoustrup; Beyerle, Andrea; Vietti, Giulia; Mortensen, Alicja; Szarek, Józef; Budtz, Hans Christian; Kermanizadeh, Ali; Banerjee, Atrayee; Ercal, Nuran; Vogel, Ulla; Wallin, Erik Håkan Richard; Møller, Peter.

In: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 85, 11.2015, p. 84-95.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Jacobsen, NR, Stoeger, T, van den Brule, S, Saber, AT, Beyerle, A, Vietti, G, Mortensen, A, Szarek, J, Budtz, HC, Kermanizadeh, A, Banerjee, A, Ercal, N, Vogel, U, Wallin, EHR & Møller, P 2015, 'Acute and subacute pulmonary toxicity and mortality in mice after intratracheal instillation of ZnO nanoparticles in three laboratories', Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, vol. 85, pp. 84-95. https://doi.org/10.1016/j.fct.2015.08.008

APA

Jacobsen, N. R., Stoeger, T., van den Brule, S., Saber, A. T., Beyerle, A., Vietti, G., Mortensen, A., Szarek, J., Budtz, H. C., Kermanizadeh, A., Banerjee, A., Ercal, N., Vogel, U., Wallin, E. H. R., & Møller, P. (2015). Acute and subacute pulmonary toxicity and mortality in mice after intratracheal instillation of ZnO nanoparticles in three laboratories. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 85, 84-95. https://doi.org/10.1016/j.fct.2015.08.008

Vancouver

Jacobsen NR, Stoeger T, van den Brule S, Saber AT, Beyerle A, Vietti G et al. Acute and subacute pulmonary toxicity and mortality in mice after intratracheal instillation of ZnO nanoparticles in three laboratories. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 2015 Nov;85:84-95. https://doi.org/10.1016/j.fct.2015.08.008

Author

Jacobsen, Nicklas Raun ; Stoeger, Tobias ; van den Brule, Sybille ; Saber, Anne Thoustrup ; Beyerle, Andrea ; Vietti, Giulia ; Mortensen, Alicja ; Szarek, Józef ; Budtz, Hans Christian ; Kermanizadeh, Ali ; Banerjee, Atrayee ; Ercal, Nuran ; Vogel, Ulla ; Wallin, Erik Håkan Richard ; Møller, Peter. / Acute and subacute pulmonary toxicity and mortality in mice after intratracheal instillation of ZnO nanoparticles in three laboratories. In: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 2015 ; Vol. 85. pp. 84-95.

Bibtex

@article{b28f78aebd094b3884727ad5f6895a23,
title = "Acute and subacute pulmonary toxicity and mortality in mice after intratracheal instillation of ZnO nanoparticles in three laboratories",
abstract = "Inhalation is the main pathway of ZnO exposure in the occupational environment but only few studies have addressed toxic effects after pulmonary exposure to ZnO nanoparticles (NP). Here we present results from three studies of pulmonary exposure and toxicity of ZnO NP in mice. The studies were prematurely terminated because interim results unexpectedly showed severe pulmonary toxicity. High bolus doses of ZnO NP (25 up to 100 μg; ≥1.4 mg/kg) were clearly associated with a dose dependent mortality in the mice. Lower doses (≥6 μg; ≥0.3 mg/kg) elicited acute toxicity in terms of reduced weight gain, desquamation of epithelial cells with concomitantly increased barrier permeability of the alveolar/blood as well as DNA damage. Oxidative stress was shown via a strong increase in lipid peroxidation and reduced glutathione in the pulmonary tissue. Two months post-exposure revealed no obvious toxicity for 12.5 and 25 μg on a range of parameters. However, mice that survived a high dose (50 μg; 2.7 mg/kg) had an increased pulmonary collagen accumulation (fibrosis) at a similar level as a high bolus dose of crystalline silica. The recovery from these toxicological effects appeared dose-dependent. The results indicate that alveolar deposition of ZnO NP may cause significant adverse health effects.",
author = "Jacobsen, {Nicklas Raun} and Tobias Stoeger and {van den Brule}, Sybille and Saber, {Anne Thoustrup} and Andrea Beyerle and Giulia Vietti and Alicja Mortensen and J{\'o}zef Szarek and Budtz, {Hans Christian} and Ali Kermanizadeh and Atrayee Banerjee and Nuran Ercal and Ulla Vogel and Wallin, {Erik H{\aa}kan Richard} and Peter M{\o}ller",
note = "Copyright {\textcopyright} 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.",
year = "2015",
month = nov,
doi = "10.1016/j.fct.2015.08.008",
language = "English",
volume = "85",
pages = "84--95",
journal = "Food and Chemical Toxicology",
issn = "0278-6915",
publisher = "Pergamon Press",

}

RIS

TY - JOUR

T1 - Acute and subacute pulmonary toxicity and mortality in mice after intratracheal instillation of ZnO nanoparticles in three laboratories

AU - Jacobsen, Nicklas Raun

AU - Stoeger, Tobias

AU - van den Brule, Sybille

AU - Saber, Anne Thoustrup

AU - Beyerle, Andrea

AU - Vietti, Giulia

AU - Mortensen, Alicja

AU - Szarek, Józef

AU - Budtz, Hans Christian

AU - Kermanizadeh, Ali

AU - Banerjee, Atrayee

AU - Ercal, Nuran

AU - Vogel, Ulla

AU - Wallin, Erik Håkan Richard

AU - Møller, Peter

N1 - Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.

PY - 2015/11

Y1 - 2015/11

N2 - Inhalation is the main pathway of ZnO exposure in the occupational environment but only few studies have addressed toxic effects after pulmonary exposure to ZnO nanoparticles (NP). Here we present results from three studies of pulmonary exposure and toxicity of ZnO NP in mice. The studies were prematurely terminated because interim results unexpectedly showed severe pulmonary toxicity. High bolus doses of ZnO NP (25 up to 100 μg; ≥1.4 mg/kg) were clearly associated with a dose dependent mortality in the mice. Lower doses (≥6 μg; ≥0.3 mg/kg) elicited acute toxicity in terms of reduced weight gain, desquamation of epithelial cells with concomitantly increased barrier permeability of the alveolar/blood as well as DNA damage. Oxidative stress was shown via a strong increase in lipid peroxidation and reduced glutathione in the pulmonary tissue. Two months post-exposure revealed no obvious toxicity for 12.5 and 25 μg on a range of parameters. However, mice that survived a high dose (50 μg; 2.7 mg/kg) had an increased pulmonary collagen accumulation (fibrosis) at a similar level as a high bolus dose of crystalline silica. The recovery from these toxicological effects appeared dose-dependent. The results indicate that alveolar deposition of ZnO NP may cause significant adverse health effects.

AB - Inhalation is the main pathway of ZnO exposure in the occupational environment but only few studies have addressed toxic effects after pulmonary exposure to ZnO nanoparticles (NP). Here we present results from three studies of pulmonary exposure and toxicity of ZnO NP in mice. The studies were prematurely terminated because interim results unexpectedly showed severe pulmonary toxicity. High bolus doses of ZnO NP (25 up to 100 μg; ≥1.4 mg/kg) were clearly associated with a dose dependent mortality in the mice. Lower doses (≥6 μg; ≥0.3 mg/kg) elicited acute toxicity in terms of reduced weight gain, desquamation of epithelial cells with concomitantly increased barrier permeability of the alveolar/blood as well as DNA damage. Oxidative stress was shown via a strong increase in lipid peroxidation and reduced glutathione in the pulmonary tissue. Two months post-exposure revealed no obvious toxicity for 12.5 and 25 μg on a range of parameters. However, mice that survived a high dose (50 μg; 2.7 mg/kg) had an increased pulmonary collagen accumulation (fibrosis) at a similar level as a high bolus dose of crystalline silica. The recovery from these toxicological effects appeared dose-dependent. The results indicate that alveolar deposition of ZnO NP may cause significant adverse health effects.

U2 - 10.1016/j.fct.2015.08.008

DO - 10.1016/j.fct.2015.08.008

M3 - Journal article

C2 - 26260750

VL - 85

SP - 84

EP - 95

JO - Food and Chemical Toxicology

JF - Food and Chemical Toxicology

SN - 0278-6915

ER -

ID: 151326762