A handsewn pericardial valved pulmonary conduit: pulsatile flow loop in vitro and acute porcine in vivo evaluation

Research output: Contribution to journalJournal articleResearchpeer-review

  • Lisa Carlson Hanse
  • Marcell Juan Tjørnild
  • Zarmiga Karunanithi
  • Johannes Høgfeldt Jedrzejczyk
  • Lejla Islamagi
  • Nynne Emilie Hummelshøj
  • Malene Enevoldsen
  • Peter Johansen
  • Mette Høj Lauridsen
  • Hjortdal, Vibeke Elisabeth

OBJECTIVES: Right ventricle to pulmonary artery anatomic discontinuity is common in complex congenital heart malformations. Handsewn conduits are a practised method of repair. In a proof-of-concept study, we evaluated pulmonary valve replacement with a handsewn pericardial valved pulmonary conduit in vitro and in vivo. METHODS: A pulsatile flow-loop model (in vitro) and an acute 60-kg porcine model (in vivo) were used. With echocardiography and pressure catheters, baseline geometry and fluid dynamics were measured. The pulmonary valve was replaced with a handsewn glutaraldehyde-treated pericardial valved pulmonary conduit corresponding to a 21-mm prosthetic valve, after which geometric measurements and fluid dynamics were reassessed. RESULTS: In vitro, 15 pulmonary trunks at 4 l/min and 13 trunks at 7 l/min, and in vivo, 11 animals were investigated. The valved pulmonary conduit was straightforward to produce at the operating table and easy to suture in place. All valves were clinically sufficient in vitro and in vivo. The mean transvalvular pressure gradient in the native valve and the conduit was 8 mmHg [standard deviation (SD): 2] and 7 mmHg (SD: 2) at 4 l/min in vitro, 19 mmHg (SD: 3) and 17 mmHg (SD: 4) at 7 l/min in vitro and 3 mmHg (SD: 2) and 6 mmHg (SD: 3) in vivo. CONCLUSIONS: Our proof-of-concept demonstrates no early evidence of structural damage to the conduit, and the fluid dynamic data were acceptable. The handsewn conduit can be produced at the operating table.

Original languageEnglish
Article numberezad143
JournalEuropean Journal of Cardio-Thoracic Surgery
Issue number4
Number of pages10
Publication statusPublished - 2023

Bibliographical note

Publisher Copyright:
© The Author(s) 2023. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved.

    Research areas

  • Cardiovascular surgery, Congenital heart disease, Echocardiography, Experimental surgery, Invasive haemodynamic, Valved pulmonary conduit

ID: 366994357