A de novo designed monomeric, compact three helix bundle protein on a carbohydrate template

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A de novo designed monomeric, compact three helix bundle protein on a carbohydrate template. / Malik, Leila; Nygård, Jesper; Christensen, Niels Johan; Madsen, Charlotte Stahl; Rösner, Heike Ilona; Kragelund, Birthe Brandt; Høiberg-Nielsen, Rasmus; Streicher, Werner; Arleth, Lise; Thulstrup, Peter Waaben; Jensen, Knud Jørgen.

In: ChemBioChem, Vol. 16, No. 13, 2015, p. 1905-1918.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Malik, L, Nygård, J, Christensen, NJ, Madsen, CS, Rösner, HI, Kragelund, BB, Høiberg-Nielsen, R, Streicher, W, Arleth, L, Thulstrup, PW & Jensen, KJ 2015, 'A de novo designed monomeric, compact three helix bundle protein on a carbohydrate template', ChemBioChem, vol. 16, no. 13, pp. 1905-1918. https://doi.org/10.1002/cbic.201500285

APA

Malik, L., Nygård, J., Christensen, N. J., Madsen, C. S., Rösner, H. I., Kragelund, B. B., Høiberg-Nielsen, R., Streicher, W., Arleth, L., Thulstrup, P. W., & Jensen, K. J. (2015). A de novo designed monomeric, compact three helix bundle protein on a carbohydrate template. ChemBioChem, 16(13), 1905-1918. https://doi.org/10.1002/cbic.201500285

Vancouver

Malik L, Nygård J, Christensen NJ, Madsen CS, Rösner HI, Kragelund BB et al. A de novo designed monomeric, compact three helix bundle protein on a carbohydrate template. ChemBioChem. 2015;16(13):1905-1918. https://doi.org/10.1002/cbic.201500285

Author

Malik, Leila ; Nygård, Jesper ; Christensen, Niels Johan ; Madsen, Charlotte Stahl ; Rösner, Heike Ilona ; Kragelund, Birthe Brandt ; Høiberg-Nielsen, Rasmus ; Streicher, Werner ; Arleth, Lise ; Thulstrup, Peter Waaben ; Jensen, Knud Jørgen. / A de novo designed monomeric, compact three helix bundle protein on a carbohydrate template. In: ChemBioChem. 2015 ; Vol. 16, No. 13. pp. 1905-1918.

Bibtex

@article{2dc6b5dfad9a4116b06755a13f25a34e,

title = "A de novo designed monomeric, compact three helix bundle protein on a carbohydrate template",

abstract = "De novo design and chemical synthesis of proteins and of other artificial structures, which mimic them, is a central strategy for understanding protein folding and for accessing proteins with novel functions. We have previously described carbohydrates as templates for the assembly of artificial proteins, so called carboproteins. The hypothesis is that the template pre-organizes the secondary structure elements and directs the formation of a tertiary structure, thus achieving structural economy in the combination of peptide, linker, and template. We speculate that the structural information from the template could facilitate protein folding. Here we report the design and synthesis of 3-helix bundle carboproteins on deoxy-hexopyranosides. The carboproteins were analyzed by CD, AUC, SAXS, and NMR, which revealed the formation of the first compact, and folded monomeric carboprotein distinctly different to a molten globule.",

author = "Leila Malik and Jesper Nyg{\aa}rd and Christensen, {Niels Johan} and Madsen, {Charlotte Stahl} and R{\"o}sner, {Heike Ilona} and Kragelund, {Birthe Brandt} and Rasmus H{\o}iberg-Nielsen and Werner Streicher and Lise Arleth and Thulstrup, {Peter Waaben} and Jensen, {Knud J{\o}rgen}",

note = "{\textcopyright} 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",

year = "2015",

doi = "10.1002/cbic.201500285",

language = "English",

volume = "16",

pages = "1905--1918",

journal = "ChemBioChem",

issn = "1439-4227",

publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",

number = "13",

}

RIS

TY - JOUR

T1 - A de novo designed monomeric, compact three helix bundle protein on a carbohydrate template

AU - Malik, Leila

AU - Nygård, Jesper

AU - Christensen, Niels Johan

AU - Madsen, Charlotte Stahl

AU - Rösner, Heike Ilona

AU - Kragelund, Birthe Brandt

AU - Høiberg-Nielsen, Rasmus

AU - Streicher, Werner

AU - Arleth, Lise

AU - Thulstrup, Peter Waaben

AU - Jensen, Knud Jørgen

PY - 2015

Y1 - 2015

N2 - De novo design and chemical synthesis of proteins and of other artificial structures, which mimic them, is a central strategy for understanding protein folding and for accessing proteins with novel functions. We have previously described carbohydrates as templates for the assembly of artificial proteins, so called carboproteins. The hypothesis is that the template pre-organizes the secondary structure elements and directs the formation of a tertiary structure, thus achieving structural economy in the combination of peptide, linker, and template. We speculate that the structural information from the template could facilitate protein folding. Here we report the design and synthesis of 3-helix bundle carboproteins on deoxy-hexopyranosides. The carboproteins were analyzed by CD, AUC, SAXS, and NMR, which revealed the formation of the first compact, and folded monomeric carboprotein distinctly different to a molten globule.

AB - De novo design and chemical synthesis of proteins and of other artificial structures, which mimic them, is a central strategy for understanding protein folding and for accessing proteins with novel functions. We have previously described carbohydrates as templates for the assembly of artificial proteins, so called carboproteins. The hypothesis is that the template pre-organizes the secondary structure elements and directs the formation of a tertiary structure, thus achieving structural economy in the combination of peptide, linker, and template. We speculate that the structural information from the template could facilitate protein folding. Here we report the design and synthesis of 3-helix bundle carboproteins on deoxy-hexopyranosides. The carboproteins were analyzed by CD, AUC, SAXS, and NMR, which revealed the formation of the first compact, and folded monomeric carboprotein distinctly different to a molten globule.

U2 - 10.1002/cbic.201500285

DO - 10.1002/cbic.201500285

M3 - Journal article

C2 - 26147795

VL - 16

SP - 1905

EP - 1918

JO - ChemBioChem

JF - ChemBioChem

SN - 1439-4227

IS - 13

ER -

ID: 141044060