Three-dimensional sperm surface reconstruction: A novel approach to assessing sperm morphology
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Three-dimensional sperm surface reconstruction : A novel approach to assessing sperm morphology. / Levine, Brian A.; Feinstein, Jeremy; Neri, Queenie V.; Goldschlag, Dan; Rosenwaks, Zev; Belongie, Serge; Palermo, Gianpiero D.
In: Fertility and Sterility, Vol. 104, No. 6, 01.12.2015, p. e14-e15.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Three-dimensional sperm surface reconstruction
T2 - A novel approach to assessing sperm morphology
AU - Levine, Brian A.
AU - Feinstein, Jeremy
AU - Neri, Queenie V.
AU - Goldschlag, Dan
AU - Rosenwaks, Zev
AU - Belongie, Serge
AU - Palermo, Gianpiero D.
N1 - Publisher Copyright: © 2015 American Society for Reproductive Medicine
PY - 2015/12/1
Y1 - 2015/12/1
N2 - Objective To create a rapid, inexpensive, efficient, and reproducible real-time three-dimensional (3-D) analysis of viable spermatozoa. Previous studies have demonstrated that abnormal semen profiles are associated with a modest increase in the frequency of sperm chromosomal abnormalities, and that sperm with aberrations in the shape and contours of the head may be carriers of chromatinic defects. Although high-power magnification and enhanced video-generated magnification have been suggested, these techniques are inherently limited by the clarity of the image, the time required for the analysis, and the risk of variable head-positioning during imaging. Design In vitro experiment. Setting University-affiliated infertility research laboratory. Patient(s) Anonymous sperm donors. Intervention(s) Individual motile sperm were identified, analyzed at ×600 magnification, and a 10-second digital video was obtained. Main Outcome Measure(s) Image-tracking software captured serial photographs of sperm from recorded videos. Images were automatically extracted from each video frame using enhanced correlation coefficient maximization; the general shape of the sperm was extracted via space-carving. The reconstructed image was rotated to permit viewing from any direction, and the final image was rendered through interpolation. Result(s) This technique yielded images that enable noninvasive, 3-D, real-time, in vitro assessment of sperm surface morphology. Conclusion(s) This proof-of-principle demonstrates that by keeping spermatozoa in a fluid environment, a 3-D sperm–surface reconstruction can be created. This technique can be automated, requires minimal computing power, and utilizes equipment already available in most embryology laboratories.
AB - Objective To create a rapid, inexpensive, efficient, and reproducible real-time three-dimensional (3-D) analysis of viable spermatozoa. Previous studies have demonstrated that abnormal semen profiles are associated with a modest increase in the frequency of sperm chromosomal abnormalities, and that sperm with aberrations in the shape and contours of the head may be carriers of chromatinic defects. Although high-power magnification and enhanced video-generated magnification have been suggested, these techniques are inherently limited by the clarity of the image, the time required for the analysis, and the risk of variable head-positioning during imaging. Design In vitro experiment. Setting University-affiliated infertility research laboratory. Patient(s) Anonymous sperm donors. Intervention(s) Individual motile sperm were identified, analyzed at ×600 magnification, and a 10-second digital video was obtained. Main Outcome Measure(s) Image-tracking software captured serial photographs of sperm from recorded videos. Images were automatically extracted from each video frame using enhanced correlation coefficient maximization; the general shape of the sperm was extracted via space-carving. The reconstructed image was rotated to permit viewing from any direction, and the final image was rendered through interpolation. Result(s) This technique yielded images that enable noninvasive, 3-D, real-time, in vitro assessment of sperm surface morphology. Conclusion(s) This proof-of-principle demonstrates that by keeping spermatozoa in a fluid environment, a 3-D sperm–surface reconstruction can be created. This technique can be automated, requires minimal computing power, and utilizes equipment already available in most embryology laboratories.
KW - noninvasive
KW - Sperm morphology
KW - three-dimensional
UR - http://www.scopus.com/inward/record.url?scp=84945539557&partnerID=8YFLogxK
U2 - 10.1016/j.fertnstert.2015.08.024
DO - 10.1016/j.fertnstert.2015.08.024
M3 - Journal article
C2 - 26363386
AN - SCOPUS:84945539557
VL - 104
SP - e14-e15
JO - Sexuality, Reproduction and Menopause
JF - Sexuality, Reproduction and Menopause
SN - 1546-2501
IS - 6
ER -
ID: 301829344