Thiol-ene Monolithic Pepsin Microreactor with a 3D-Printed Interface for Efficient UPLC-MS Peptide Mapping Analyses
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Thiol-ene Monolithic Pepsin Microreactor with a 3D-Printed Interface for Efficient UPLC-MS Peptide Mapping Analyses. / Jönsson, Alexander; Svejdal, Rasmus R; Bøgelund, Nanna; Nguyen, Tam T T N; Flindt, Henrik; Kutter, Jörg P; Rand, Kasper D; Lafleur, Josiane P.
In: Analytical Chemistry, Vol. 89, No. 8, 21.03.2017, p. 4573-4580.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Thiol-ene Monolithic Pepsin Microreactor with a 3D-Printed Interface for Efficient UPLC-MS Peptide Mapping Analyses
AU - Jönsson, Alexander
AU - Svejdal, Rasmus R
AU - Bøgelund, Nanna
AU - Nguyen, Tam T T N
AU - Flindt, Henrik
AU - Kutter, Jörg P
AU - Rand, Kasper D
AU - Lafleur, Josiane P
PY - 2017/3/21
Y1 - 2017/3/21
N2 - To improve the sample handling, and reduce cost and preparation time, of peptide mapping LC-MS workflows in protein analytical research, we here investigate the possibility of replacing conventional enzymatic digestion methods with a polymer microfluidic chip based enzyme reactor. Off-stoichiometric thiol-ene is utilized as both bulk material and as a monolithic stationary phase for immobilization of the proteolytic enzyme pepsin. The digestion efficiency of the, thiol-ene based, immobilized enzyme reactor (IMER) is compared to that of a conventional, agarose packed bed, pepsin IMER column commonly used in LC-MS based protein analyses. The chip IMER is found to rival the conventional column in terms of digestion efficiency at comparable residence time and, using a 3D-printed interface, be directly interfaceable with LC-MS.
AB - To improve the sample handling, and reduce cost and preparation time, of peptide mapping LC-MS workflows in protein analytical research, we here investigate the possibility of replacing conventional enzymatic digestion methods with a polymer microfluidic chip based enzyme reactor. Off-stoichiometric thiol-ene is utilized as both bulk material and as a monolithic stationary phase for immobilization of the proteolytic enzyme pepsin. The digestion efficiency of the, thiol-ene based, immobilized enzyme reactor (IMER) is compared to that of a conventional, agarose packed bed, pepsin IMER column commonly used in LC-MS based protein analyses. The chip IMER is found to rival the conventional column in terms of digestion efficiency at comparable residence time and, using a 3D-printed interface, be directly interfaceable with LC-MS.
KW - Journal Article
U2 - 10.1021/acs.analchem.6b05103
DO - 10.1021/acs.analchem.6b05103
M3 - Journal article
C2 - 28322047
VL - 89
SP - 4573
EP - 4580
JO - Industrial And Engineering Chemistry Analytical Edition
JF - Industrial And Engineering Chemistry Analytical Edition
SN - 0003-2700
IS - 8
ER -
ID: 176596936