The relation between red meat and whole-grain intake and the colonic mucosal barrier: A cross-sectional study
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The relation between red meat and whole-grain intake and the colonic mucosal barrier : A cross-sectional study. / Jawhara, Mohamad; Sørensen, Signe Bek; Heitmann, Berit Lilienthal; Halldórsson, Þórhallur Ingi; Kristian Pedersen, Andreas; Andersen, Vibeke.
In: Nutrients, Vol. 12, No. 6, 1765, 2020, p. 1-18.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The relation between red meat and whole-grain intake and the colonic mucosal barrier
T2 - A cross-sectional study
AU - Jawhara, Mohamad
AU - Sørensen, Signe Bek
AU - Heitmann, Berit Lilienthal
AU - Halldórsson, Þórhallur Ingi
AU - Kristian Pedersen, Andreas
AU - Andersen, Vibeke
PY - 2020
Y1 - 2020
N2 - The Colonic Mucosal Barrier (CMB) is the site of interaction between the human body and the colonic microbiota. The mucus is the outer part of the CMB and is considered as the front-line defense of the colon. It separates the host epithelial lining from the colonic content, and it has previously been linked to health and diseases. In this study, we assessed the relationship between red meat and whole-grain intake and (1) the thickness of the colonic mucus (2) the expression of the predominant mucin gene in the human colon (MUC2). Patients referred to colonoscopy at the University Hospital of Southern Denmark-Sonderjylland were enrolled between June 2017 and December 2018, and lifestyle data was collected in a cross-sectional study design. Colonic biopsies, blood, urine, and fecal samples were collected. The colonic mucus and bacteria were visualized by immunostaining and fluorescence in situ hybridization techniques. We found a thinner mucus was associated with high red meat intake. Similarly, the results suggested a thinner mucus was associated with high whole-grain intake, albeit to a lesser extent than red meat. This is the first study assessing the association between red meat and whole-grain intake and the colonic mucus in humans. This study is approved by the Danish Ethics Committee (S-20160124) and the Danish Data Protecting Agency (2008-58-035). A study protocol was registered at clinical trials.gov under NCT04235348.
AB - The Colonic Mucosal Barrier (CMB) is the site of interaction between the human body and the colonic microbiota. The mucus is the outer part of the CMB and is considered as the front-line defense of the colon. It separates the host epithelial lining from the colonic content, and it has previously been linked to health and diseases. In this study, we assessed the relationship between red meat and whole-grain intake and (1) the thickness of the colonic mucus (2) the expression of the predominant mucin gene in the human colon (MUC2). Patients referred to colonoscopy at the University Hospital of Southern Denmark-Sonderjylland were enrolled between June 2017 and December 2018, and lifestyle data was collected in a cross-sectional study design. Colonic biopsies, blood, urine, and fecal samples were collected. The colonic mucus and bacteria were visualized by immunostaining and fluorescence in situ hybridization techniques. We found a thinner mucus was associated with high red meat intake. Similarly, the results suggested a thinner mucus was associated with high whole-grain intake, albeit to a lesser extent than red meat. This is the first study assessing the association between red meat and whole-grain intake and the colonic mucus in humans. This study is approved by the Danish Ethics Committee (S-20160124) and the Danish Data Protecting Agency (2008-58-035). A study protocol was registered at clinical trials.gov under NCT04235348.
KW - Colonic mucosal barrier
KW - Human colon
KW - Large intestine
KW - MUC2
KW - Mucin
KW - Mucus
KW - Red meat
KW - Whole-grain
U2 - 10.3390/nu12061765
DO - 10.3390/nu12061765
M3 - Journal article
C2 - 32545531
AN - SCOPUS:85086432235
VL - 12
SP - 1
EP - 18
JO - Nutrients
JF - Nutrients
SN - 2072-6643
IS - 6
M1 - 1765
ER -
ID: 245370366