The impact of PrsA over-expression on the Bacillus subtilis transcriptome during fed-batch fermentation of alpha-amylase production
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The impact of PrsA over-expression on the Bacillus subtilis transcriptome during fed-batch fermentation of alpha-amylase production. / Geissler, Adrian S.; Poulsen, Line D.; Doncheva, Nadezhda T.; Anthon, Christian; Seemann, Stefan E.; González-Tortuero, Enrique; Breüner, Anne; Jensen, Lars J.; Hjort, Carsten; Vinther, Jeppe; Gorodkin, Jan.
In: Frontiers in Microbiology, Vol. 13, 909493, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The impact of PrsA over-expression on the Bacillus subtilis transcriptome during fed-batch fermentation of alpha-amylase production
AU - Geissler, Adrian S.
AU - Poulsen, Line D.
AU - Doncheva, Nadezhda T.
AU - Anthon, Christian
AU - Seemann, Stefan E.
AU - González-Tortuero, Enrique
AU - Breüner, Anne
AU - Jensen, Lars J.
AU - Hjort, Carsten
AU - Vinther, Jeppe
AU - Gorodkin, Jan
N1 - Publisher Copyright: Copyright © 2022 Geissler, Poulsen, Doncheva, Anthon, Seemann, González-Tortuero, Breüner, Jensen, Hjort, Vinther and Gorodkin.
PY - 2022
Y1 - 2022
N2 - The production of the alpha-amylase (AMY) enzyme in Bacillus subtilis at a high rate leads to the accumulation of unfolded AMY, which causes secretion stress. The over-expression of the PrsA chaperone aids enzyme folding and reduces stress. To identify affected pathways and potential mechanisms involved in the reduced growth, we analyzed the transcriptomic differences during fed-batch fermentation between a PrsA over-expressing strain and control in a time-series RNA-seq experiment. We observe transcription in 542 unannotated regions, of which 234 had significant changes in expression levels between the samples. Moreover, 1,791 protein-coding sequences, 80 non-coding genes, and 20 riboswitches overlapping UTR regions of coding genes had significant changes in expression. We identified putatively regulated biological processes via gene-set over-representation analysis of the differentially expressed genes; overall, the analysis suggests that the PrsA over-expression affects ATP biosynthesis activity, amino acid metabolism, and cell wall stability. The investigation of the protein interaction network points to a potential impact on cell motility signaling. We discuss the impact of these highlighted mechanisms for reducing secretion stress or detrimental aspects of PrsA over-expression during AMY production.
AB - The production of the alpha-amylase (AMY) enzyme in Bacillus subtilis at a high rate leads to the accumulation of unfolded AMY, which causes secretion stress. The over-expression of the PrsA chaperone aids enzyme folding and reduces stress. To identify affected pathways and potential mechanisms involved in the reduced growth, we analyzed the transcriptomic differences during fed-batch fermentation between a PrsA over-expressing strain and control in a time-series RNA-seq experiment. We observe transcription in 542 unannotated regions, of which 234 had significant changes in expression levels between the samples. Moreover, 1,791 protein-coding sequences, 80 non-coding genes, and 20 riboswitches overlapping UTR regions of coding genes had significant changes in expression. We identified putatively regulated biological processes via gene-set over-representation analysis of the differentially expressed genes; overall, the analysis suggests that the PrsA over-expression affects ATP biosynthesis activity, amino acid metabolism, and cell wall stability. The investigation of the protein interaction network points to a potential impact on cell motility signaling. We discuss the impact of these highlighted mechanisms for reducing secretion stress or detrimental aspects of PrsA over-expression during AMY production.
KW - alpha-amylase
KW - Bacillus subtilis
KW - enzyme produced by microorganism
KW - PrsA
KW - RNA sequencing (RNA-seq)
U2 - 10.3389/fmicb.2022.909493
DO - 10.3389/fmicb.2022.909493
M3 - Journal article
C2 - 35992681
AN - SCOPUS:85136486443
VL - 13
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
SN - 1664-302X
M1 - 909493
ER -
ID: 318801289