The EUROFORGEN NAME Ampliseq™ custom panel: A second tier panel developed for differentiation of individuals from the Middle East/North Africa
Research output: Contribution to journal › Journal article › Research › peer-review
The interest in ancestry informative markers as an investigative tool to identify the biogeographical ancestry of potential suspects is increasing. For this purpose, many different panels have been designed to distinguish individuals from major geographical regions. The EUROFORGEN North Africa and Middle East ancestry panel is a second-tier panel designed for differentiating Middle Eastern/North African populations from other population groups. An Ion AmpliSeq™ custom panel (Thermo Fisher Scientific) was designed to sequence populations of interest using the Ion S5™ (Thermo Fisher Scientific). Of the 111 SNPs selected in the first version of the panel, 107 SNPs were successfully incorporated in the AmpliSeq™ multiplex PCR. More than 600 individuals from 13 different countries in the Middle East, North Africa, and Europe were typed. The performance of the sequencing assay was assessed by calculating relative locus balances, heterozygote balances, and noise levels. Lastly, likelihood ratios were calculated using the GenoGeographer software.
Original language | English |
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Journal | Forensic Science International: Genetics Supplement Series |
Volume | 7 |
Issue number | 1 |
Pages (from-to) | 846-848 |
ISSN | 1875-1768 |
DOIs | |
Publication status | Published - Dec 2019 |
- Ancestry informative markers, EUROFORGEN NAME ancestry panel, Ion AmpliSeq™ custom panel, Ion S5™ sequencing, NAME, Second-tier panel
Research areas
ID: 234506163