The effect of long-term treatment with granulocyte colony-stimulating factor on hematopoiesis in HIV-infected individuals
Research output: Contribution to journal › Journal article › Research › peer-review
This randomized, placebo-controlled trial examine the long-term effect of granulocyte colony-stimulating factor (G-CSF) on absolute numbers of CD34+ progenitor cells and progenitor cell function in human immunodeficiency virus (HIV)-infected patients. G-CSF (300 microg filgrastim) or placebo was given three times weekly for 12 weeks to 30 HIV-infected patients that had been treated with HAART for at least 24 weeks and not yet achieved CD4 counts above 350 CD4+ cells/microl. Blood samples were collected at weeks 0, 2, 4, 8, and 12, and again 12 weeks after termination of the G-CSF treatment. Significant increase in absolute numbers of circulating CD34+ cells was detected in the treatment group (P = 0.006). The function of progenitor cells was examined in vitro using a colony-forming unit (CFU) assay, and increase in the number of CFU/ml was detected (P = 0.005). In order to estimate the effect of G-CSF on in vivo function of progenitors the white-blood count was determined. Significant increase in white-blood count was found (P < 0.001), while hemoglobin and platelet count decreased (P = 0.001 and P = 0.013, respectively). Significant increase in the CD4 count occurred, but correlation between the numbers of progenitors and the CD4 count was not found. These data suggest that G-CSF mainly increases the number and differentiation of myeloid progenitors.
Original language | English |
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Journal | Scandinavian Journal of Immunology |
Volume | 52 |
Issue number | 3 |
Pages (from-to) | 298-303 |
Number of pages | 6 |
ISSN | 0300-9475 |
Publication status | Published - Sep 2000 |
- Anti-HIV Agents, Blood Cell Count, CD4 Lymphocyte Count, Cell Differentiation, Female, Filgrastim, Granulocyte Colony-Stimulating Factor, HIV Infections, Hematopoiesis, Hematopoietic Stem Cell Mobilization, Hemoglobins, Humans, Leukocyte Count, Male, Neutropenia, Platelet Count, Recombinant Proteins, Time Factors, Clinical Trial, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't
Research areas
ID: 180571797