Synergistic permeability enhancing effect of lysophospholipids and fatty acids on lipid membranes
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Synergistic permeability enhancing effect of lysophospholipids and fatty acids on lipid membranes. / Davidsen, Jesper; Mouritsen, Ole G.; Jorgensen, Kent.
In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1564, No. 1, 19.08.2002, p. 256-262.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Synergistic permeability enhancing effect of lysophospholipids and fatty acids on lipid membranes
AU - Davidsen, Jesper
AU - Mouritsen, Ole G.
AU - Jorgensen, Kent
PY - 2002/8/19
Y1 - 2002/8/19
N2 - The permeability-enhancing effects of the two surfactants, 1-palmitoyl-2-lyso-sn-gycero-3-phosphocholine (lysoPPC) and palmitic acid (PA), on lipid membranes that at physiological temperatures are in the gel, fluid, and liquid-ordered phases were determined using the concentration-dependent self-quenching properties of the hydrophilic marker, calcein. Adding lysoPPC to lipid membranes in the gel-phase induced a time-dependent calcein release curve that can be described by the sum of two exponentials, whereas PA induces a considerably more complex release curve. However, when lysoPPC and PA were added simultaneously in equimolar concentrations, a dramatic synergistic permeability-enhancing effect was observed. In contrast, when both lysoPPC and PA are added to liposomal membranes that are in the fluid or liquid-ordered phases, no effect on the transmembrane permeation of calcein was observed.
AB - The permeability-enhancing effects of the two surfactants, 1-palmitoyl-2-lyso-sn-gycero-3-phosphocholine (lysoPPC) and palmitic acid (PA), on lipid membranes that at physiological temperatures are in the gel, fluid, and liquid-ordered phases were determined using the concentration-dependent self-quenching properties of the hydrophilic marker, calcein. Adding lysoPPC to lipid membranes in the gel-phase induced a time-dependent calcein release curve that can be described by the sum of two exponentials, whereas PA induces a considerably more complex release curve. However, when lysoPPC and PA were added simultaneously in equimolar concentrations, a dramatic synergistic permeability-enhancing effect was observed. In contrast, when both lysoPPC and PA are added to liposomal membranes that are in the fluid or liquid-ordered phases, no effect on the transmembrane permeation of calcein was observed.
KW - Drug-delivery
KW - Enhancer
KW - Fatty acid
KW - Liposome
KW - Lysophospholipid
KW - Membrane
KW - Permeability
KW - Surfactant
UR - http://www.scopus.com/inward/record.url?scp=0037136068&partnerID=8YFLogxK
U2 - 10.1016/S0005-2736(02)00461-3
DO - 10.1016/S0005-2736(02)00461-3
M3 - Journal article
C2 - 12101020
AN - SCOPUS:0037136068
VL - 1564
SP - 256
EP - 262
JO - B B A - Biomembranes
JF - B B A - Biomembranes
SN - 0005-2736
IS - 1
ER -
ID: 230987051