State-of-the-art glycosaminoglycan characterization

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State-of-the-art glycosaminoglycan characterization. / Zappe, Andreas; Miller, Rebecca L.; Struwe, Weston B.; Pagel, Kevin.

In: Mass Spectrometry Reviews, Vol. 41, No. 6, 2022, p. 1040-1071.

Research output: Contribution to journal › Review › Research › peer-review

Harvard

Zappe, A, Miller, RL, Struwe, WB & Pagel, K 2022, 'State-of-the-art glycosaminoglycan characterization', Mass Spectrometry Reviews, vol. 41, no. 6, pp. 1040-1071. https://doi.org/10.1002/mas.21737

APA

Zappe, A., Miller, R. L., Struwe, W. B., & Pagel, K. (2022). State-of-the-art glycosaminoglycan characterization. Mass Spectrometry Reviews, 41(6), 1040-1071. https://doi.org/10.1002/mas.21737

Vancouver

Zappe A, Miller RL, Struwe WB, Pagel K. State-of-the-art glycosaminoglycan characterization. Mass Spectrometry Reviews. 2022;41(6):1040-1071. https://doi.org/10.1002/mas.21737

Author

Zappe, Andreas ; Miller, Rebecca L. ; Struwe, Weston B. ; Pagel, Kevin. / State-of-the-art glycosaminoglycan characterization. In: Mass Spectrometry Reviews. 2022 ; Vol. 41, No. 6. pp. 1040-1071.

Bibtex

@article{664b6c0c706246b591d16f561803cbb9,

title = "State-of-the-art glycosaminoglycan characterization",

abstract = "Glycosaminoglycans (GAGs) are heterogeneous acidic polysaccharides involved in a range of biological functions. They have a significant influence on the regulation of cellular processes and the development of various diseases and infections. To fully understand the functional roles that GAGs play in mammalian systems, including disease processes, it is essential to understand their structural features. Despite having a linear structure and a repetitive disaccharide backbone, their structural analysis is challenging and requires elaborate preparative and analytical techniques. In particular, the extent to which GAGs are sulfated, as well as variation in sulfate position across the entire oligosaccharide or on individual monosaccharides, represents a major obstacle. Here, we summarize the current state-of-the-art methodologies used for GAG sample preparation and analysis, discussing in detail liquid chromatograpy and mass spectrometry-based approaches, including advanced ion activation methods, ion mobility separations and infrared action spectroscopy of mass-selected species.",

keywords = "gas-phase spectroscopy, glycosaminoglycans, infrared spectroscopy, ion mobility spectrometry, liquid chromatography, mass spectrometry",

author = "Andreas Zappe and Miller, {Rebecca L.} and Struwe, {Weston B.} and Kevin Pagel",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors. Mass Spectrometry Reviews published by John Wiley & Sons Ltd.",

year = "2022",

doi = "10.1002/mas.21737",

language = "English",

volume = "41",

pages = "1040--1071",

journal = "Mass Spectrometry Reviews",

issn = "0277-7037",

publisher = "JohnWiley & Sons, Inc.",

number = "6",

}

RIS

TY - JOUR

T1 - State-of-the-art glycosaminoglycan characterization

AU - Zappe, Andreas

AU - Miller, Rebecca L.

AU - Struwe, Weston B.

AU - Pagel, Kevin

PY - 2022

Y1 - 2022

N2 - Glycosaminoglycans (GAGs) are heterogeneous acidic polysaccharides involved in a range of biological functions. They have a significant influence on the regulation of cellular processes and the development of various diseases and infections. To fully understand the functional roles that GAGs play in mammalian systems, including disease processes, it is essential to understand their structural features. Despite having a linear structure and a repetitive disaccharide backbone, their structural analysis is challenging and requires elaborate preparative and analytical techniques. In particular, the extent to which GAGs are sulfated, as well as variation in sulfate position across the entire oligosaccharide or on individual monosaccharides, represents a major obstacle. Here, we summarize the current state-of-the-art methodologies used for GAG sample preparation and analysis, discussing in detail liquid chromatograpy and mass spectrometry-based approaches, including advanced ion activation methods, ion mobility separations and infrared action spectroscopy of mass-selected species.

AB - Glycosaminoglycans (GAGs) are heterogeneous acidic polysaccharides involved in a range of biological functions. They have a significant influence on the regulation of cellular processes and the development of various diseases and infections. To fully understand the functional roles that GAGs play in mammalian systems, including disease processes, it is essential to understand their structural features. Despite having a linear structure and a repetitive disaccharide backbone, their structural analysis is challenging and requires elaborate preparative and analytical techniques. In particular, the extent to which GAGs are sulfated, as well as variation in sulfate position across the entire oligosaccharide or on individual monosaccharides, represents a major obstacle. Here, we summarize the current state-of-the-art methodologies used for GAG sample preparation and analysis, discussing in detail liquid chromatograpy and mass spectrometry-based approaches, including advanced ion activation methods, ion mobility separations and infrared action spectroscopy of mass-selected species.

KW - gas-phase spectroscopy

KW - glycosaminoglycans

KW - infrared spectroscopy

KW - ion mobility spectrometry

KW - liquid chromatography

KW - mass spectrometry

U2 - 10.1002/mas.21737

DO - 10.1002/mas.21737

M3 - Review

C2 - 34608657

AN - SCOPUS:85116341946

VL - 41

SP - 1040

EP - 1071

JO - Mass Spectrometry Reviews

JF - Mass Spectrometry Reviews

SN - 0277-7037

IS - 6

ER -

ID: 284178757