TY - JOUR

T1 - Staphylococcus aureus isolates from bovine mastitis in eight countries

T2 - Genotypes, detection of genes encoding different toxins and other virulence genes

AU - Monistero, Valentina

AU - Graber, Hans Ulrich

AU - Pollera, Claudia

AU - Cremonesi, Paola

AU - Castiglioni, Bianca

AU - Bottini, Enriqueta

AU - Ceballos-Marquez, Alejandro

AU - Lasso-Rojas, Laura

AU - Kroemker, Volker

AU - Wente, Nicole

AU - Petzer, Inge Marie

AU - Santisteban, Carlos

AU - Runyan, Jeff

AU - dos Santos, Marcos Veiga

AU - Alves, Bruna Gomes

AU - Piccinini, Renata

AU - Bronzo, Valerio

AU - Abbassi, Mohamed Salah

AU - Said, Meriam Ben

AU - Moroni, Paolo

N1 - Funding Information: Department of Veterinary Medicine, University of Milan, via Celoria 10, 20133 Milan, Italy; valentina.monistero@gmail.com (V.M.); claudia.pollera@unimi.it (C.P.); renata.piccinini@unimi.it (R.P.); valerio.bronzo@unimi.it (V.B.); paolo.moroni@unimi.it (P.M.) Agroscope, Research Division, Food Microbial Systems, Schwarzenburgstrasse 161, 3003 Bern, Switzerland; hansulrich.graber@agroscope.admin.ch Institute of Agricultural Biology and Biotechnology, National Research Council, via Einstein, 26900 Lodi, Italy; casti@ibba.cnr.it Laboratorio de Microbiologia Clinica y Experimental, Departamento de Sanidad Animal y Medicina Preventiva SAMP/CIVENTAN, Becaria CONICET, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (FCV, UNCPBA), Paraje Arroyo Seco S/N, Campus Universitario, CP 7000 Tandil, Buenos Aires, Argentina; bottini.enriqueta@gmail.com Laboratorio de Calidad de Leche y Epidemiología Veterinaria (Grupo CLEV), Universidad de Caldas, Calle 65 #26-10, Manizales, Caldas, Colombia; alejandro.ceballos@ucaldas.edu.co (A.C.-M.); laura.lasso.mvz@gmail.com (L.L.-R.) Bioprocess Engineering—Faculty II, University of Applied Sciences and Arts, Microbiology Heisterbergallee 12, 30453 Hannover, Germany; Volker.Kroemker@hs-hannover.de (V.K.); nicole.wente@hs-hannover.de (N.W.) Faculty of Veterinary Science, University of Pretoria, M35, Pretoria 0110, South Africa; Inge-Marie.Petzer@up.ac.za Quality Milk Production Services, Animal Health Diagnostic Center, Cornell University, 240 Farrier Road, Ithaca, NY 14850, USA; cgs1@cornell.edu (C.S.); jpr253@cornell.edu (J.R.) Department of Animal Nutrition and Production, School of Veterinary Medicine and Animal Sciences, Rua Duque de Caxias Norte, 225, Pirassununga-SP 13635900, Brazil; mveiga@usp.br (M.V.d.S.); bgalves@usp.br (B.G.A.) 10 Tunisian Institute of Veterinary Research, University of Tunis El Manar, Tunis 1068, Tunisia; salahtoumi_mohamed@yahoo.com (M.S.A.); mbs-mariem@hotmail.fr (M.B.S.) Publisher Copyright: © 2018 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2018/6/17

Y1 - 2018/6/17

N2 - Staphylococcus aureus is recognized worldwide as one of the major agents of dairy cow intra-mammary infections. This microorganism can express a wide spectrum of pathogenic factors used to attach, colonize, invade and infect the host. The present study evaluated 120 isolates from eight different countries that were genotyped by RS-PCR and investigated for 26 different virulence factors to increase the knowledge on the circulating genetic lineages among the cow population with mastitis. New genotypes were observed for South African strains while for all the other countries new variants of existing genotypes were detected. For each country, a specific genotypic pattern was found. Among the virulence factors, fmtB, cna, clfA and leucocidins genes were the most frequent. The sea and sei genes were present in seven out of eight countries; seh showed high frequency in South American countries (Brazil, Colombia, Argentina), while sel was harboured especially in one Mediterranean country (Tunisia). The etb, seb and see genes were not detected in any of the isolates, while only two isolates were MRSA (Germany and Italy) confirming the low diffusion of methicillin resistance microorganism among bovine mastitis isolates. This work demonstrated the wide variety of S. aureus genotypes found in dairy cattle worldwide. This condition suggests that considering the region of interest might help to formulate strategies for reducing the infection spreading.

AB - Staphylococcus aureus is recognized worldwide as one of the major agents of dairy cow intra-mammary infections. This microorganism can express a wide spectrum of pathogenic factors used to attach, colonize, invade and infect the host. The present study evaluated 120 isolates from eight different countries that were genotyped by RS-PCR and investigated for 26 different virulence factors to increase the knowledge on the circulating genetic lineages among the cow population with mastitis. New genotypes were observed for South African strains while for all the other countries new variants of existing genotypes were detected. For each country, a specific genotypic pattern was found. Among the virulence factors, fmtB, cna, clfA and leucocidins genes were the most frequent. The sea and sei genes were present in seven out of eight countries; seh showed high frequency in South American countries (Brazil, Colombia, Argentina), while sel was harboured especially in one Mediterranean country (Tunisia). The etb, seb and see genes were not detected in any of the isolates, while only two isolates were MRSA (Germany and Italy) confirming the low diffusion of methicillin resistance microorganism among bovine mastitis isolates. This work demonstrated the wide variety of S. aureus genotypes found in dairy cattle worldwide. This condition suggests that considering the region of interest might help to formulate strategies for reducing the infection spreading.

KW - Dairy cow

KW - Genotypes

KW - Mastitis

KW - S. aureus

KW - Virulence genes

U2 - 10.3390/toxins10060247

DO - 10.3390/toxins10060247

M3 - Journal article

C2 - 29914197

AN - SCOPUS:85048726796

VL - 10

JO - Toxins

JF - Toxins

SN - 2072-6651

IS - 6

M1 - 247

ER -