Skin barrier damage after exposure to paraphenylenediamine

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Skin barrier damage after exposure to paraphenylenediamine. / Meisser, Sanne S; Altunbulakli, Can; Bandier, Josefine; Opstrup, Morten S; Castro-Giner, Francesc; Akdis, Mübeccel; Bonefeld, Charlotte M; Johansen, Jeanne D; Akdis, Cezmi A.

In: The Journal of allergy and clinical immunology, Vol. 145, No. 2, 2020, p. 619-631.e2.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Meisser, SS, Altunbulakli, C, Bandier, J, Opstrup, MS, Castro-Giner, F, Akdis, M, Bonefeld, CM, Johansen, JD & Akdis, CA 2020, 'Skin barrier damage after exposure to paraphenylenediamine', The Journal of allergy and clinical immunology, vol. 145, no. 2, pp. 619-631.e2. https://doi.org/10.1016/j.jaci.2019.11.023

APA

Meisser, S. S., Altunbulakli, C., Bandier, J., Opstrup, M. S., Castro-Giner, F., Akdis, M., Bonefeld, C. M., Johansen, J. D., & Akdis, C. A. (2020). Skin barrier damage after exposure to paraphenylenediamine. The Journal of allergy and clinical immunology, 145(2), 619-631.e2. https://doi.org/10.1016/j.jaci.2019.11.023

Vancouver

Meisser SS, Altunbulakli C, Bandier J, Opstrup MS, Castro-Giner F, Akdis M et al. Skin barrier damage after exposure to paraphenylenediamine. The Journal of allergy and clinical immunology. 2020;145(2):619-631.e2. https://doi.org/10.1016/j.jaci.2019.11.023

Author

Meisser, Sanne S ; Altunbulakli, Can ; Bandier, Josefine ; Opstrup, Morten S ; Castro-Giner, Francesc ; Akdis, Mübeccel ; Bonefeld, Charlotte M ; Johansen, Jeanne D ; Akdis, Cezmi A. / Skin barrier damage after exposure to paraphenylenediamine. In: The Journal of allergy and clinical immunology. 2020 ; Vol. 145, No. 2. pp. 619-631.e2.

Bibtex

@article{ae263620369348609c4631a2a1f3962f,

title = "Skin barrier damage after exposure to paraphenylenediamine",

abstract = "BACKGROUND: p-Phenylenediamine (PPD) is a strong contact allergen used in hair dye that is known to cause allergic contact dermatitis (ACD). Both private and occupational exposure to PPD is frequent, but the effect of PPD exposure in nonallergic occupationally exposed subjects is unknown.OBJECTIVE: We sought to investigate the effects of PPD exposure on the skin of occupationally exposed subjects with and without clinical symptoms.METHODS: Skin biopsy specimens were collected from 4 patients with mild and 5 patients with severe PPD-related ACD and 7 hairdressers without contact dermatitis on day 4 after patch testing with 1% PPD in petrolatum. RNA sequencing and transcriptomics analyses were performed and confirmed by using quantitative RT-PCR. Protein expression was analyzed in skin from 4 hairdressers and 1 patient with ACD by using immunofluorescence staining. Reconstructed human epidermis was used to test the effects of PPD in vitro.RESULTS: RNA sequencing demonstrated downregulation of tight junction and stratum corneum proteins in the skin of patients with severe ACD after PPD exposure. Claudin-1 (CLDN-1), CLDN8, CLDN11, CXADR-like membrane protein (CLMP), occludin (OCLN), membrane-associated guanylate kinase inverted 1 (MAGI1), and MAGI2 mRNA expression was downregulated in patients with severe ACD. CLDN1 and CLMP expression were downregulated in nonresponding hairdressers and patients with mild ACD. Filaggrin 1 (FLG1), FLG2, and loricrin (LOR) expression were downregulated in patients with ACD. Confocal microscopic images showed downregulation of CLDN-1, FLG-1, and FLG-2 expression. In contrast, 3-dimensional skin cultures showed upregulation of FLG-1 in response to PPD but downregulation of FLG-2.CONCLUSION: PPD-exposed skin is associated with extensive transcriptomic changes, including downregulation of tight junction and stratum corneum proteins, even in the absence of clinical symptoms.",

keywords = "Adult, Dermatitis, Allergic Contact/etiology, Female, Hair Dyes/adverse effects, Humans, Occupational Exposure/adverse effects, Phenylenediamines/adverse effects, Skin/drug effects, Tight Junction Proteins/drug effects",

author = "Meisser, {Sanne S} and Can Altunbulakli and Josefine Bandier and Opstrup, {Morten S} and Francesc Castro-Giner and M{\"u}beccel Akdis and Bonefeld, {Charlotte M} and Johansen, {Jeanne D} and Akdis, {Cezmi A}",

year = "2020",

doi = "10.1016/j.jaci.2019.11.023",

language = "English",

volume = "145",

pages = "619--631.e2",

journal = "Journal of Allergy and Clinical Immunology",

issn = "0091-6749",

publisher = "Mosby Inc.",

number = "2",

}

RIS

TY - JOUR

T1 - Skin barrier damage after exposure to paraphenylenediamine

AU - Meisser, Sanne S

AU - Altunbulakli, Can

AU - Bandier, Josefine

AU - Opstrup, Morten S

AU - Castro-Giner, Francesc

AU - Akdis, Mübeccel

AU - Bonefeld, Charlotte M

AU - Johansen, Jeanne D

AU - Akdis, Cezmi A

PY - 2020

Y1 - 2020

N2 - BACKGROUND: p-Phenylenediamine (PPD) is a strong contact allergen used in hair dye that is known to cause allergic contact dermatitis (ACD). Both private and occupational exposure to PPD is frequent, but the effect of PPD exposure in nonallergic occupationally exposed subjects is unknown.OBJECTIVE: We sought to investigate the effects of PPD exposure on the skin of occupationally exposed subjects with and without clinical symptoms.METHODS: Skin biopsy specimens were collected from 4 patients with mild and 5 patients with severe PPD-related ACD and 7 hairdressers without contact dermatitis on day 4 after patch testing with 1% PPD in petrolatum. RNA sequencing and transcriptomics analyses were performed and confirmed by using quantitative RT-PCR. Protein expression was analyzed in skin from 4 hairdressers and 1 patient with ACD by using immunofluorescence staining. Reconstructed human epidermis was used to test the effects of PPD in vitro.RESULTS: RNA sequencing demonstrated downregulation of tight junction and stratum corneum proteins in the skin of patients with severe ACD after PPD exposure. Claudin-1 (CLDN-1), CLDN8, CLDN11, CXADR-like membrane protein (CLMP), occludin (OCLN), membrane-associated guanylate kinase inverted 1 (MAGI1), and MAGI2 mRNA expression was downregulated in patients with severe ACD. CLDN1 and CLMP expression were downregulated in nonresponding hairdressers and patients with mild ACD. Filaggrin 1 (FLG1), FLG2, and loricrin (LOR) expression were downregulated in patients with ACD. Confocal microscopic images showed downregulation of CLDN-1, FLG-1, and FLG-2 expression. In contrast, 3-dimensional skin cultures showed upregulation of FLG-1 in response to PPD but downregulation of FLG-2.CONCLUSION: PPD-exposed skin is associated with extensive transcriptomic changes, including downregulation of tight junction and stratum corneum proteins, even in the absence of clinical symptoms.

AB - BACKGROUND: p-Phenylenediamine (PPD) is a strong contact allergen used in hair dye that is known to cause allergic contact dermatitis (ACD). Both private and occupational exposure to PPD is frequent, but the effect of PPD exposure in nonallergic occupationally exposed subjects is unknown.OBJECTIVE: We sought to investigate the effects of PPD exposure on the skin of occupationally exposed subjects with and without clinical symptoms.METHODS: Skin biopsy specimens were collected from 4 patients with mild and 5 patients with severe PPD-related ACD and 7 hairdressers without contact dermatitis on day 4 after patch testing with 1% PPD in petrolatum. RNA sequencing and transcriptomics analyses were performed and confirmed by using quantitative RT-PCR. Protein expression was analyzed in skin from 4 hairdressers and 1 patient with ACD by using immunofluorescence staining. Reconstructed human epidermis was used to test the effects of PPD in vitro.RESULTS: RNA sequencing demonstrated downregulation of tight junction and stratum corneum proteins in the skin of patients with severe ACD after PPD exposure. Claudin-1 (CLDN-1), CLDN8, CLDN11, CXADR-like membrane protein (CLMP), occludin (OCLN), membrane-associated guanylate kinase inverted 1 (MAGI1), and MAGI2 mRNA expression was downregulated in patients with severe ACD. CLDN1 and CLMP expression were downregulated in nonresponding hairdressers and patients with mild ACD. Filaggrin 1 (FLG1), FLG2, and loricrin (LOR) expression were downregulated in patients with ACD. Confocal microscopic images showed downregulation of CLDN-1, FLG-1, and FLG-2 expression. In contrast, 3-dimensional skin cultures showed upregulation of FLG-1 in response to PPD but downregulation of FLG-2.CONCLUSION: PPD-exposed skin is associated with extensive transcriptomic changes, including downregulation of tight junction and stratum corneum proteins, even in the absence of clinical symptoms.

KW - Adult

KW - Dermatitis, Allergic Contact/etiology

KW - Female

KW - Hair Dyes/adverse effects

KW - Humans

KW - Occupational Exposure/adverse effects

KW - Phenylenediamines/adverse effects

KW - Skin/drug effects

KW - Tight Junction Proteins/drug effects

U2 - 10.1016/j.jaci.2019.11.023

DO - 10.1016/j.jaci.2019.11.023

M3 - Journal article

C2 - 31783056

VL - 145

SP - 619-631.e2

JO - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

SN - 0091-6749

IS - 2

ER -

ID: 257659298