Single-Cell RNA Sequencing of the T Helper Cell Response to House Dust Mites Defines a Distinct Gene Expression Signature in Airway Th2 Cells
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Single-Cell RNA Sequencing of the T Helper Cell Response to House Dust Mites Defines a Distinct Gene Expression Signature in Airway Th2 Cells. / Tibbitt, Christopher Andrew; Stark, Julian Mario; Martens, Liesbet; Ma, Junjie; Mold, Jeff Eron; Deswarte, Kim; Oliynyk, Ganna; Feng, Xiaogang; Lambrecht, Bart Norbert; De Bleser, Pieter; Nylén, Susanne; Hammad, Hamida; Arsenian Henriksson, Marie; Saeys, Yvan; Coquet, Jonathan Marie.
In: Immunity, Vol. 51, No. 1, 16.07.2019, p. 169-184.e5.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Single-Cell RNA Sequencing of the T Helper Cell Response to House Dust Mites Defines a Distinct Gene Expression Signature in Airway Th2 Cells
AU - Tibbitt, Christopher Andrew
AU - Stark, Julian Mario
AU - Martens, Liesbet
AU - Ma, Junjie
AU - Mold, Jeff Eron
AU - Deswarte, Kim
AU - Oliynyk, Ganna
AU - Feng, Xiaogang
AU - Lambrecht, Bart Norbert
AU - De Bleser, Pieter
AU - Nylén, Susanne
AU - Hammad, Hamida
AU - Arsenian Henriksson, Marie
AU - Saeys, Yvan
AU - Coquet, Jonathan Marie
N1 - Copyright © 2019 Elsevier Inc. All rights reserved.
PY - 2019/7/16
Y1 - 2019/7/16
N2 - Naive CD4+ T cells differentiate into functionally diverse T helper (Th) cell subsets. Th2 cells play a pathogenic role in asthma, yet a clear picture of their transcriptional profile is lacking. We performed single-cell RNA sequencing (scRNA-seq) of T helper cells from lymph node, lung, and airways in the house dust mite (HDM) model of allergic airway disease. scRNA-seq resolved transcriptional profiles of naive CD4+ T, Th1, Th2, regulatory T (Treg) cells, and a CD4+ T cell population responsive to type I interferons. Th2 cells in the airways were enriched for transcription of many genes, including Cd200r1, Il6, Plac8, and Igfbp7, and their mRNA profile was supported by analysis of chromatin accessibility and flow cytometry. Pathways associated with lipid metabolism were enriched in Th2 cells, and experiments with inhibitors of key metabolic pathways supported roles for glucose and lipid metabolism. These findings provide insight into the differentiation of pathogenic Th2 cells in the context of allergy.
AB - Naive CD4+ T cells differentiate into functionally diverse T helper (Th) cell subsets. Th2 cells play a pathogenic role in asthma, yet a clear picture of their transcriptional profile is lacking. We performed single-cell RNA sequencing (scRNA-seq) of T helper cells from lymph node, lung, and airways in the house dust mite (HDM) model of allergic airway disease. scRNA-seq resolved transcriptional profiles of naive CD4+ T, Th1, Th2, regulatory T (Treg) cells, and a CD4+ T cell population responsive to type I interferons. Th2 cells in the airways were enriched for transcription of many genes, including Cd200r1, Il6, Plac8, and Igfbp7, and their mRNA profile was supported by analysis of chromatin accessibility and flow cytometry. Pathways associated with lipid metabolism were enriched in Th2 cells, and experiments with inhibitors of key metabolic pathways supported roles for glucose and lipid metabolism. These findings provide insight into the differentiation of pathogenic Th2 cells in the context of allergy.
KW - Animals
KW - Antigens, Dermatophagoides/immunology
KW - Asthma/immunology
KW - Disease Models, Animal
KW - Humans
KW - Lipid Metabolism/genetics
KW - Mice
KW - Mice, Inbred C57BL
KW - Mice, Knockout
KW - Orexin Receptors/genetics
KW - Pyroglyphidae/immunology
KW - Respiratory Hypersensitivity/immunology
KW - Respiratory System/immunology
KW - Sequence Analysis, RNA
KW - Single-Cell Analysis
KW - T-Lymphocyte Subsets/immunology
KW - Th2 Cells/immunology
KW - Transcriptome
U2 - 10.1016/j.immuni.2019.05.014
DO - 10.1016/j.immuni.2019.05.014
M3 - Journal article
C2 - 31231035
VL - 51
SP - 169-184.e5
JO - Immunity
JF - Immunity
SN - 1074-7613
IS - 1
ER -
ID: 356968648