Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices. / Lafleur, Josiane P.; Jensen, T.G.; Kutter, Jörg P.; Kwapiszewski, R.

In: Analyst, Vol. 138, No. 3, 07.02.2013, p. 845-849.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Lafleur, JP, Jensen, TG, Kutter, JP & Kwapiszewski, R 2013, 'Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices', Analyst, vol. 138, no. 3, pp. 845-849. https://doi.org/10.1039/c2an36424g

APA

Lafleur, J. P., Jensen, T. G., Kutter, J. P., & Kwapiszewski, R. (2013). Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices. Analyst, 138(3), 845-849. https://doi.org/10.1039/c2an36424g

Vancouver

Lafleur JP, Jensen TG, Kutter JP, Kwapiszewski R. Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices. Analyst. 2013 Feb 7;138(3):845-849. https://doi.org/10.1039/c2an36424g

Author

Lafleur, Josiane P. ; Jensen, T.G. ; Kutter, Jörg P. ; Kwapiszewski, R. / Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices. In: Analyst. 2013 ; Vol. 138, No. 3. pp. 845-849.

Bibtex

@article{6de29d7c70284bf990e44785ff060f7f,
title = "Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices",
abstract = "The suitable optical properties of thiol-ene polymers combined with the ease of modifying their surface for the attachment of recognition molecules make them ideal candidates in many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in microfluidic thiol-ene chips. This work focuses on thiol-ene substrates featuring an excess of thiol groups at their surface. The thiol-ene stoichiometric composition can be varied to precisely control the number of surface thiol groups available for surface modification up to an average surface density of 136 ± 17 SH nm(-2). Biotin alkyne was patterned directly inside thiol-ene microchannels prior to conjugation with fluorescently labelled streptavidin. The surface bound conjugates were detected by evanescent wave-induced fluorescence (EWIF), demonstrating the success of the grafting procedure and its potential for biochip applications.",
keywords = "Alkynes, Biotin, Fluorescent Dyes, Microfluidic Analytical Techniques, Polymers, Spectrometry, Fluorescence, Streptavidin, Sulfhydryl Compounds, Surface Properties",
author = "Lafleur, {Josiane P.} and T.G. Jensen and Kutter, {J{\"o}rg P.} and R. Kwapiszewski",
year = "2013",
month = feb,
day = "7",
doi = "10.1039/c2an36424g",
language = "English",
volume = "138",
pages = "845--849",
journal = "The Analyst",
issn = "0003-2654",
publisher = "Royal Society of Chemistry",
number = "3",

}

RIS

TY - JOUR

T1 - Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices

AU - Lafleur, Josiane P.

AU - Jensen, T.G.

AU - Kutter, Jörg P.

AU - Kwapiszewski, R.

PY - 2013/2/7

Y1 - 2013/2/7

N2 - The suitable optical properties of thiol-ene polymers combined with the ease of modifying their surface for the attachment of recognition molecules make them ideal candidates in many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in microfluidic thiol-ene chips. This work focuses on thiol-ene substrates featuring an excess of thiol groups at their surface. The thiol-ene stoichiometric composition can be varied to precisely control the number of surface thiol groups available for surface modification up to an average surface density of 136 ± 17 SH nm(-2). Biotin alkyne was patterned directly inside thiol-ene microchannels prior to conjugation with fluorescently labelled streptavidin. The surface bound conjugates were detected by evanescent wave-induced fluorescence (EWIF), demonstrating the success of the grafting procedure and its potential for biochip applications.

AB - The suitable optical properties of thiol-ene polymers combined with the ease of modifying their surface for the attachment of recognition molecules make them ideal candidates in many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in microfluidic thiol-ene chips. This work focuses on thiol-ene substrates featuring an excess of thiol groups at their surface. The thiol-ene stoichiometric composition can be varied to precisely control the number of surface thiol groups available for surface modification up to an average surface density of 136 ± 17 SH nm(-2). Biotin alkyne was patterned directly inside thiol-ene microchannels prior to conjugation with fluorescently labelled streptavidin. The surface bound conjugates were detected by evanescent wave-induced fluorescence (EWIF), demonstrating the success of the grafting procedure and its potential for biochip applications.

KW - Alkynes

KW - Biotin

KW - Fluorescent Dyes

KW - Microfluidic Analytical Techniques

KW - Polymers

KW - Spectrometry, Fluorescence

KW - Streptavidin

KW - Sulfhydryl Compounds

KW - Surface Properties

UR - http://www.scopus.com/inward/record.url?scp=84872092079&partnerID=8YFLogxK

U2 - 10.1039/c2an36424g

DO - 10.1039/c2an36424g

M3 - Journal article

C2 - 23193537

AN - SCOPUS:84872092079

VL - 138

SP - 845

EP - 849

JO - The Analyst

JF - The Analyst

SN - 0003-2654

IS - 3

ER -

ID: 50586256