Protocol for quantifying pyramidal neuron hyperexcitability in a mouse model of neurodevelopmental encephalopathy
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Protocol for quantifying pyramidal neuron hyperexcitability in a mouse model of neurodevelopmental encephalopathy. / dos Santos, Altair Brito; Larsen, Silas Dalum; Gomez, Carlos Daniel; Sørensen, Jakob Balslev; Perrier, Jean François.
In: STAR Protocols, Vol. 5, No. 2, 102954, 2024.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Protocol for quantifying pyramidal neuron hyperexcitability in a mouse model of neurodevelopmental encephalopathy
AU - dos Santos, Altair Brito
AU - Larsen, Silas Dalum
AU - Gomez, Carlos Daniel
AU - Sørensen, Jakob Balslev
AU - Perrier, Jean François
N1 - Publisher Copyright: © 2024 The Authors
PY - 2024
Y1 - 2024
N2 - Here, we present a protocol for quantifying pyramidal neuron hyperexcitability in a mouse model of STXBP1 neurodevelopmental encephalopathy (Stxbp1hap). We describe steps for preparing brain slices, positioning electrodes, and performing an excitability test to investigate microcircuit failures. This protocol is based on recording layer 2/3 cortical pyramidal neurons in response to stimulation of two independent sets of excitatory axons that recruit feedforward inhibition microcircuits. For complete details on the use and execution of this protocol, please refer to Dos Santos et al.1
AB - Here, we present a protocol for quantifying pyramidal neuron hyperexcitability in a mouse model of STXBP1 neurodevelopmental encephalopathy (Stxbp1hap). We describe steps for preparing brain slices, positioning electrodes, and performing an excitability test to investigate microcircuit failures. This protocol is based on recording layer 2/3 cortical pyramidal neurons in response to stimulation of two independent sets of excitatory axons that recruit feedforward inhibition microcircuits. For complete details on the use and execution of this protocol, please refer to Dos Santos et al.1
KW - Genetics
KW - Health Sciences
KW - Neuroscience
U2 - 10.1016/j.xpro.2024.102954
DO - 10.1016/j.xpro.2024.102954
M3 - Journal article
C2 - 38492227
AN - SCOPUS:85188189040
VL - 5
JO - STAR Protocols
JF - STAR Protocols
SN - 2666-1667
IS - 2
M1 - 102954
ER -
ID: 387269165