Proliferation and apoptosis of lamina propria CD4+ T cells from scid mice with inflammatory bowel disease
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Scid mice transplanted with low numbers of syngeneic CD4+ T cells, develop a chronic and lethal inflammatory bowel disease (IBD) within 4-6 months. We have used in vivo 5-bromo2-deoxy-uridine (BrdU) labeling to assess the proliferation of lamina propria-derived CD4+ T cells in diseased scid mice. The hourly rate of renewal of colonic lamina propria CD4+ T cells in diseased mice was 7% compared with 1.5% in normal BALB/c control mice. Transplantation of scid mice with in vitro activated CD4+ T cells accelerated the disease onset and development in a cell dose-dependent fashion when compared with non-activated CD4+ T cells. In pulse-chase experiments it was shown that BrdU-labeled cells disappeared rapidly from the lamina propria of diseased mice. DNA analysis revealed that this was due to the presence of nearly four times as many apoptotic CD4+ T cells in diseased than in control mice. Further analyses showed that the apoptotic lamina propria CD4+ T cells were derived from cells having entered the cell cycle within the previous 8 h. These data clearly demonstrate that vigorous CD4+ T cell proliferation and death are involved throughout the course of IBD.
Original language | English |
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Journal | European Journal of Immunology |
Volume | 28 |
Issue number | 11 |
Pages (from-to) | 3655-63 |
Number of pages | 9 |
ISSN | 0014-2980 |
DOIs | |
Publication status | Published - 1 Nov 1998 |
- Animals, Apoptosis, Bromodeoxyuridine, CD4-Positive T-Lymphocytes, Inflammatory Bowel Diseases, Intestinal Mucosa, Lymphocyte Activation, Mice, Mice, Inbred BALB C, Mice, SCID, Rats
Research areas
ID: 32637467