Profiling of GABAA and GABAB receptor expression in the myometrium of the human uterus
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Profiling of GABAA and GABAB receptor expression in the myometrium of the human uterus. / Söderhielm, Pella Cecilia; Klein, Anders Bue; Bomholtz, Sofia Hammami; Jensen, Anders A.
In: Life Sciences, Vol. 214, 2018, p. 145-152.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Profiling of GABAA and GABAB receptor expression in the myometrium of the human uterus
AU - Söderhielm, Pella Cecilia
AU - Klein, Anders Bue
AU - Bomholtz, Sofia Hammami
AU - Jensen, Anders A.
PY - 2018
Y1 - 2018
N2 - AbstractAims: gamma-aminobutyric acid (GABA) mediates its physiological effects through the GABA(A) and GABA(B) receptors. In this study the putative expression of GABA(A)R and GABA(B)R subunits in human myometrium tissue was investigated. Main methods: The expression levels of the 19 GABA(A)R subunits (alpha(1)-alpha(6),beta(1)-beta(3), gamma(1)-gamma(3), delta, epsilon, pi, theta, rho(1)-rho(3)) and the three GABA(B)R subunits (GABA(B1a), GABA(B1b), GABA(B2)) were characterized by RT-qPCR analysis on two commercial samples and six samples derived from surgically removed myometrial tissues from different women. We probed for functional GABA(A)R expression in primary human myometrial smooth muscle cells (HMSMCs) by whole-cell patch-clamp electrophysiology. Key findings: The absolute mRNA levels of the 22 GABA(A)R and GABA(B)R genes varied considerably across the eight samples, but a pronounced overlap existed between the specific subunits detected in the samples, with alpha(2), beta(2) , beta(3), epsilon, pi, theta, GABA(B1a) and GABA(B1b) mRNAs being detected in most samples. The expression profile of GABA(A)R and GABA(B)R subunit mRNAs in HMSMCs correlated with that observed in the eight tissue samples, albeit the subunit transcripts were detected at lower relative levels. Neither muscimol nor GABA evoked significant currents in these cells in the patch-clamp recordings. Significance: While the expression of the GABA(B1) subunits on their own is unlikely to give rise to functional GABA(B)R expression, the GABA(A)R subunits identified at mRNA level would be able to form functional receptors in the human myometrial tissue. Although GABA(A)R-mediated currents could not be recorded from HMSMCs in this study, this suggests a role for GABAergic transmission in the human myometrium
AB - AbstractAims: gamma-aminobutyric acid (GABA) mediates its physiological effects through the GABA(A) and GABA(B) receptors. In this study the putative expression of GABA(A)R and GABA(B)R subunits in human myometrium tissue was investigated. Main methods: The expression levels of the 19 GABA(A)R subunits (alpha(1)-alpha(6),beta(1)-beta(3), gamma(1)-gamma(3), delta, epsilon, pi, theta, rho(1)-rho(3)) and the three GABA(B)R subunits (GABA(B1a), GABA(B1b), GABA(B2)) were characterized by RT-qPCR analysis on two commercial samples and six samples derived from surgically removed myometrial tissues from different women. We probed for functional GABA(A)R expression in primary human myometrial smooth muscle cells (HMSMCs) by whole-cell patch-clamp electrophysiology. Key findings: The absolute mRNA levels of the 22 GABA(A)R and GABA(B)R genes varied considerably across the eight samples, but a pronounced overlap existed between the specific subunits detected in the samples, with alpha(2), beta(2) , beta(3), epsilon, pi, theta, GABA(B1a) and GABA(B1b) mRNAs being detected in most samples. The expression profile of GABA(A)R and GABA(B)R subunit mRNAs in HMSMCs correlated with that observed in the eight tissue samples, albeit the subunit transcripts were detected at lower relative levels. Neither muscimol nor GABA evoked significant currents in these cells in the patch-clamp recordings. Significance: While the expression of the GABA(B1) subunits on their own is unlikely to give rise to functional GABA(B)R expression, the GABA(A)R subunits identified at mRNA level would be able to form functional receptors in the human myometrial tissue. Although GABA(A)R-mediated currents could not be recorded from HMSMCs in this study, this suggests a role for GABAergic transmission in the human myometrium
U2 - 10.1016/j.lfs.2018.10.033
DO - 10.1016/j.lfs.2018.10.033
M3 - Journal article
C2 - 30343129
VL - 214
SP - 145
EP - 152
JO - Life Sciences
JF - Life Sciences
SN - 0024-3205
ER -
ID: 203600293