Probiotics Support Resilience of the Oral Microbiota during Resolution after Experimental Gingivitis - A Randomized, Double-Blinded, Placebo-Controlled Trial
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Probiotics Support Resilience of the Oral Microbiota during Resolution after Experimental Gingivitis - A Randomized, Double-Blinded, Placebo-Controlled Trial. / Olsen, Christine Lundtorp; Massarenti, Laura; Vendius, Vincent Frederik Dahl; Gürsoy, Ulvi Kahraman; Van Splunter, Annina; Bikker, Floris J.; Gürsoy, Mervi; Damgaard, Christian; Markvart, Merete; Belstrøm, Daniel.
In: Nutrients, Vol. 15, No. 22, 4805, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Probiotics Support Resilience of the Oral Microbiota during Resolution after Experimental Gingivitis - A Randomized, Double-Blinded, Placebo-Controlled Trial
AU - Olsen, Christine Lundtorp
AU - Massarenti, Laura
AU - Vendius, Vincent Frederik Dahl
AU - Gürsoy, Ulvi Kahraman
AU - Van Splunter, Annina
AU - Bikker, Floris J.
AU - Gürsoy, Mervi
AU - Damgaard, Christian
AU - Markvart, Merete
AU - Belstrøm, Daniel
PY - 2023
Y1 - 2023
N2 - The present study aims to test whether probiotics protect against experimental gingivitis incited by 14 days of oral hygiene neglect and/or subsequently support the restoration of oral homeostasis. Eighty systemically and orally healthy participants refrained from oral hygiene procedures for 14 days, followed by 14 days with regular oral hygiene procedures. Additionally, participants consumed either probiotics ( n = 40) or placebo ( n = 40) throughout the trial. At baseline, day 14, and day 28, supragingival plaque score and bleeding-on-probing percentage (BOP %) were registered, and supragingival plaque and saliva samples were collected. The supragingival microbiota was characterized using 16S sequencing, and saliva samples were analyzed for levels of pro-inflammatory cytokines and proteases. At day 28, the relative abundance of Lautropia ( p = 0.014), Prevotella ( p = 0.046), Fusobacterium ( p = 0.033), and Selenomonas ( p = 0.0078) genera were significantly higher in the placebo group compared to the probiotics group, while the relative abundance of Rothia ( p = 0.047) species was associated with the probiotics group. Streptococcus sanguinis was associated with the probiotics group, while Campylobacter gracilis was associated with the placebo group. No difference was observed in salivary cytokines, albumin, or any enzyme activity. The present study suggests that probiotics support the resilience of the oral microbiota in the resolution period after gingivitis.
AB - The present study aims to test whether probiotics protect against experimental gingivitis incited by 14 days of oral hygiene neglect and/or subsequently support the restoration of oral homeostasis. Eighty systemically and orally healthy participants refrained from oral hygiene procedures for 14 days, followed by 14 days with regular oral hygiene procedures. Additionally, participants consumed either probiotics ( n = 40) or placebo ( n = 40) throughout the trial. At baseline, day 14, and day 28, supragingival plaque score and bleeding-on-probing percentage (BOP %) were registered, and supragingival plaque and saliva samples were collected. The supragingival microbiota was characterized using 16S sequencing, and saliva samples were analyzed for levels of pro-inflammatory cytokines and proteases. At day 28, the relative abundance of Lautropia ( p = 0.014), Prevotella ( p = 0.046), Fusobacterium ( p = 0.033), and Selenomonas ( p = 0.0078) genera were significantly higher in the placebo group compared to the probiotics group, while the relative abundance of Rothia ( p = 0.047) species was associated with the probiotics group. Streptococcus sanguinis was associated with the probiotics group, while Campylobacter gracilis was associated with the placebo group. No difference was observed in salivary cytokines, albumin, or any enzyme activity. The present study suggests that probiotics support the resilience of the oral microbiota in the resolution period after gingivitis.
KW - Humans
KW - Gingivitis/therapy
KW - Microbiota
KW - Research Design
KW - Probiotics/therapeutic use
KW - Cytokines
U2 - 10.3390/nu15224805
DO - 10.3390/nu15224805
M3 - Journal article
C2 - 38004199
VL - 15
JO - Nutrients
JF - Nutrients
SN - 2072-6643
IS - 22
M1 - 4805
ER -
ID: 375059348