Plant nonsense-mediated mRNA decay is controlled by different autoregulatory circuits and can be induced by an EJC-like complex
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Plant nonsense-mediated mRNA decay is controlled by different autoregulatory circuits and can be induced by an EJC-like complex. / Nyikó, Tünde; Kerényi, Farkas; Szabadkai, Levente; Benkovics, Anna H.; Major, Péter; Sonkoly, Boglárka; Mérai, Zsuzsanna; Barta, Endre; Niemiec, Emilia; Kufel, Joanna; Silhavy, Dániel.
In: Nucleic Acids Research, Vol. 41, No. 13, 07.2013, p. 6715-6728.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Plant nonsense-mediated mRNA decay is controlled by different autoregulatory circuits and can be induced by an EJC-like complex
AU - Nyikó, Tünde
AU - Kerényi, Farkas
AU - Szabadkai, Levente
AU - Benkovics, Anna H.
AU - Major, Péter
AU - Sonkoly, Boglárka
AU - Mérai, Zsuzsanna
AU - Barta, Endre
AU - Niemiec, Emilia
AU - Kufel, Joanna
AU - Silhavy, Dániel
N1 - Funding Information: Hungarian Scientific Research Fund [OTKA K60102, C77086]; International Centre for Genetic Engineering and Biotechnology [ICGEB CRP/HUN09-01]. T.N. and L.S. were graduate students of the ELTE ‘Classical and Molecular Genetics’ PhD program. EMBO short term fellowship program (to T.N.) and the Marie-Curie [PIEFGA-2009-253075] fellowship program (to Z.M.). Funding for open access charge: Grants from Hungarian Scientific Research Fund [OTKA K60102, C77086 and ICGEB CRP/HUN09-01] will be used to pay for the publication charge.
PY - 2013/7
Y1 - 2013/7
N2 - Nonsense-mediated mRNA decay (NMD) is a eukaryotic quality control system that recognizes and degrades transcripts containing NMD cis elements in their 3′untranslated region (UTR). In yeasts, unusually long 3′UTRs act as NMD cis elements, whereas in vertebrates, NMD is induced by introns located >50 nt downstream from the stop codon. In vertebrates, splicing leads to deposition of exon junction complex (EJC) onto the mRNA, and then 3′UTR-bound EJCs trigger NMD. It is proposed that this intron-based NMD is vertebrate specific, and it evolved to eliminate the misproducts of alternative splicing. Here, we provide evidence that similar EJC-mediated intron-based NMD functions in plants, suggesting that this type of NMD is evolutionary conserved. We demonstrate that in plants, like in vertebrates, introns located >50 nt from the stop induces NMD. We show that orthologs of all core EJC components are essential for intron-based plant NMD and that plant Partner of Y14 and mago (PYM) also acts as EJC disassembly factor. Moreover, we found that complex autoregulatory circuits control the activity of plant NMD. We demonstrate that expression of suppressor with morphogenic effect on genitalia (SMG)7, which is essential for long 3′UTR- and intron-based NMD, is regulated by both types of NMD, whereas expression of Barentsz EJC component is downregulated by intron-based NMD.
AB - Nonsense-mediated mRNA decay (NMD) is a eukaryotic quality control system that recognizes and degrades transcripts containing NMD cis elements in their 3′untranslated region (UTR). In yeasts, unusually long 3′UTRs act as NMD cis elements, whereas in vertebrates, NMD is induced by introns located >50 nt downstream from the stop codon. In vertebrates, splicing leads to deposition of exon junction complex (EJC) onto the mRNA, and then 3′UTR-bound EJCs trigger NMD. It is proposed that this intron-based NMD is vertebrate specific, and it evolved to eliminate the misproducts of alternative splicing. Here, we provide evidence that similar EJC-mediated intron-based NMD functions in plants, suggesting that this type of NMD is evolutionary conserved. We demonstrate that in plants, like in vertebrates, introns located >50 nt from the stop induces NMD. We show that orthologs of all core EJC components are essential for intron-based plant NMD and that plant Partner of Y14 and mago (PYM) also acts as EJC disassembly factor. Moreover, we found that complex autoregulatory circuits control the activity of plant NMD. We demonstrate that expression of suppressor with morphogenic effect on genitalia (SMG)7, which is essential for long 3′UTR- and intron-based NMD, is regulated by both types of NMD, whereas expression of Barentsz EJC component is downregulated by intron-based NMD.
UR - http://www.scopus.com/inward/record.url?scp=84880534583&partnerID=8YFLogxK
U2 - 10.1093/nar/gkt366
DO - 10.1093/nar/gkt366
M3 - Journal article
C2 - 23666629
AN - SCOPUS:84880534583
VL - 41
SP - 6715
EP - 6728
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 13
ER -
ID: 369542517