Onset of transcription of the aminopeptidase N (leukemia antigen CD 13) gene at the crypt/villus transition zone during rabbit enterocyte differentiation.
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The sequence of a cDNA clone (2.82 kbp) of rabbit intestinal aminopeptidase N (CD 13) is reported. Using the corresponding anti-sense RNA probe, the distribution of aminopeptidase N mRNA along the crypt/villus axis of the rabbit small intestine was studied by in situ hybridization. The aminopeptidase N gene is expressed along the whole length of the villus with a maximum at its base. Expression was not detected in the crypt cells. The distribution of aminopeptidase N mRNA correlates with the presence of active enzyme as monitored by histochemical staining. The results are compatible with onset of transcription of the aminopeptidase N gene at the crypt/villus transition zone during the enterocyte differentiation.
Original language | English |
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Journal | FEBS Letters |
Volume | 259 |
Issue number | 1 |
Pages (from-to) | 107-12 |
Number of pages | 5 |
ISSN | 0014-5793 |
Publication status | Published - 1989 |
Bibliographical note
Keywords: Amino Acid Sequence; Aminopeptidases; Animals; Antigens, CD13; Antigens, Differentiation, Myelomonocytic; Base Sequence; Blotting, Northern; Cell Differentiation; Gene Expression Regulation, Enzymologic; Intestine, Small; Microvilli; Molecular Sequence Data; Nucleic Acid Hybridization; RNA Probes; Rabbits; Transcription, Genetic
ID: 6586536