No additional effect of different types of physical activity on 10-hour muscle protein synthesis in elderly men on a controlled energy- and protein-sufficient diet
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
No additional effect of different types of physical activity on 10-hour muscle protein synthesis in elderly men on a controlled energy- and protein-sufficient diet. / Bulow, Jacob; Agergaard, Jakob; Kjær, Michael; Holm, Lars; Reitelseder, Soren.
In: Experimental Gerontology, Vol. 79, 15.06.2016, p. 16-25.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - No additional effect of different types of physical activity on 10-hour muscle protein synthesis in elderly men on a controlled energy- and protein-sufficient diet
AU - Bulow, Jacob
AU - Agergaard, Jakob
AU - Kjær, Michael
AU - Holm, Lars
AU - Reitelseder, Soren
PY - 2016/6/15
Y1 - 2016/6/15
N2 - Purpose: The elderly lose skeletal muscle mass with age, which may be detrimental for function and quality of life. Both inactivity and heavy resistance exercise are known to have marked but opposite effects upon muscle mass. However, the potential effects of daily physical activity upon muscle protein synthesis (MPS) are less investigated. The aim of this study was to determine the effects of daily physical activities upon MPS in elderly individuals.Methods: A total of 24 elderly men (70 +/- 1 year) were recruited and randomly assigned: inactivity in form of bed-rest (IA), daily physical activities (DA), or heavy resistance exercise (RE). All groups undertook a normal eating routine containing carbohydrates (52 E%), fat (32 E%), and protein (16 E%). Ingestion of labeled milk protein ([1-C-13] leucine-labeled whey and caseinate) served to maintain tracer enrichment for determination of 10-hour myofibrillar protein fractional synthesis rates (FSR), and typical prerequisites for calculating FSR were fulfilled. Physical activities were monitored, and venous blood and muscle biopsies collected.Results: Physical activity was highest in the DA compared to both the IA and RE groups. Nutrient ingestion increased insulin, leucine, and phenylalanine plasma concentrations in all groups. [1-C-13] leucine enrichment was stable throughout the 10-hour FSR period. Myofibrillar protein FSR were similar for IA, DA, and RE groups, 0.055 +/- 0.003%/h, 0.058 +/- 0.006%/h, and 0.065 +/- 0.008%/h, respectively (means +/- SE, P = 0.44).Conclusions: In elderly males, inactivity, daily activities, and resistance exercise interventions result in equal 10-hour, whole day MPS during an energy-and protein-sufficient diet regimen. (C) 2016 Elsevier Inc. All rights reserved.
AB - Purpose: The elderly lose skeletal muscle mass with age, which may be detrimental for function and quality of life. Both inactivity and heavy resistance exercise are known to have marked but opposite effects upon muscle mass. However, the potential effects of daily physical activity upon muscle protein synthesis (MPS) are less investigated. The aim of this study was to determine the effects of daily physical activities upon MPS in elderly individuals.Methods: A total of 24 elderly men (70 +/- 1 year) were recruited and randomly assigned: inactivity in form of bed-rest (IA), daily physical activities (DA), or heavy resistance exercise (RE). All groups undertook a normal eating routine containing carbohydrates (52 E%), fat (32 E%), and protein (16 E%). Ingestion of labeled milk protein ([1-C-13] leucine-labeled whey and caseinate) served to maintain tracer enrichment for determination of 10-hour myofibrillar protein fractional synthesis rates (FSR), and typical prerequisites for calculating FSR were fulfilled. Physical activities were monitored, and venous blood and muscle biopsies collected.Results: Physical activity was highest in the DA compared to both the IA and RE groups. Nutrient ingestion increased insulin, leucine, and phenylalanine plasma concentrations in all groups. [1-C-13] leucine enrichment was stable throughout the 10-hour FSR period. Myofibrillar protein FSR were similar for IA, DA, and RE groups, 0.055 +/- 0.003%/h, 0.058 +/- 0.006%/h, and 0.065 +/- 0.008%/h, respectively (means +/- SE, P = 0.44).Conclusions: In elderly males, inactivity, daily activities, and resistance exercise interventions result in equal 10-hour, whole day MPS during an energy-and protein-sufficient diet regimen. (C) 2016 Elsevier Inc. All rights reserved.
KW - Intrinsically labeled milk protein tracer
KW - Stable isotope
KW - Muscle protein synthesis
KW - Fractional synthesis rate
KW - Activities of daily living
U2 - 10.1016/j.exger.2016.03.008
DO - 10.1016/j.exger.2016.03.008
M3 - Journal article
C2 - 26979470
VL - 79
SP - 16
EP - 25
JO - Experimental Gerontology
JF - Experimental Gerontology
SN - 0531-5565
ER -
ID: 166499932