NADPH oxidase is internalized by clathrin-coated pits and localizes to a Rab27A/B GTPase-regulated secretory compartment in activated macrophages
Research output: Contribution to journal › Journal article › Research › peer-review
Here, we report that activation of different types of tissue macrophages, including microglia, by lipopolysaccharide (LPS) or GM-CSF stimulation correlates with the quantitative redistribution of NADPH oxidase (cyt b(558)) from the plasma membrane to an intracellular stimulus-responsive storage compartment. Cryo-immunogold labeling of gp91(phox) and CeCl(3) cytochemistry showed the presence of gp91(phox) and oxidant production in numerous small (
| Original language | English |
|---|---|
| Journal | Journal of Biological Chemistry |
| Volume | 287 |
| Issue number | 7 |
| Pages (from-to) | 4835-52 |
| Number of pages | 18 |
| ISSN | 0021-9258 |
| DOIs | |
| Publication status | Published - Feb 2012 |
- Adenosine Triphosphate, Animals, CD40 Ligand, Calcium-Binding Proteins, Cells, Cultured, Clathrin, Clathrin-Coated Vesicles, Cytochrome b Group, Endosomes, Exocytosis, Intracellular Signaling Peptides and Proteins, Macrophage Activation, Macrophages, Membrane Glycoproteins, Microglia, NADPH Oxidase, Rats, Superoxides, Tumor Necrosis Factor-alpha, rab GTP-Binding Proteins
Research areas
ID: 38406486