Mutation-Guided Unbiased Modeling of the Fat Sensor GPR119 for High-Yield Agonist Screening.
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Recent benchmark studies have demonstrated the difficulties in obtaining accurate predictions of ligand binding conformations to comparative models of G-protein-coupled receptors. We have developed a data-driven optimization protocol, which integrates mutational data and structural information from multiple X-ray receptor structures in combination with a fully flexible ligand docking protocol to det. the binding conformation of AR231453, a small-mol. agonist, in the GPR119 receptor. Resulting models converge to one conformation that explains the majority of data from mutation studies and is consistent with the structure-activity relationship for a large no. of AR231453 analogs. Another key property of the refined models is their success in sepg. active ligands from decoys in a large-scale virtual screening. These results demonstrate that mutation-guided receptor modeling can provide predictions of practical value for describing receptor-ligand interactions and drug discovery.
Original language | English |
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Journal | Structure |
Volume | 23 |
Issue number | 12 |
Pages (from-to) | 2377-2386 |
Number of pages | 10 |
ISSN | 0969-2126 |
DOIs | |
Publication status | Published - 2015 |
Bibliographical note
M1 - Copyright (C) 2015 American Chemical Society (ACS). All Rights Reserved.
CAPLUS AN 2015:1756764(Journal; Online Computer File)
ID: 150703222