Morphological and Genetic Diversity of Trichuris spp. recovered from Humans and Pigs

Research output: Chapter in Book/Report/Conference proceeding › Conference abstract in proceedings › Research

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Morphological and Genetic Diversity of Trichuris spp. recovered from Humans and Pigs. / Nissen, Sofie; Nejsum, Peter; Christensen, Henrik; Olsen, Annette; Thamsborg, Stig Milan.

 One World, one Health - Parasites in a changing landscape. 2009. p. 109-110.

Research output: Chapter in Book/Report/Conference proceeding › Conference abstract in proceedings › Research

Harvard

Nissen, S, Nejsum, P, Christensen, H, Olsen, A & Thamsborg, SM 2009, Morphological and Genetic Diversity of Trichuris spp. recovered from Humans and Pigs. in  One World, one Health - Parasites in a changing landscape. pp. 109-110, World Association for the Advancement of Veterinary Parasitology, Calgary, Canada, 09/08/2009.

APA

Nissen, S., Nejsum, P., Christensen, H., Olsen, A., & Thamsborg, S. M. (2009). Morphological and Genetic Diversity of Trichuris spp. recovered from Humans and Pigs. In  One World, one Health - Parasites in a changing landscape (pp. 109-110)

Vancouver

Nissen S, Nejsum P, Christensen H, Olsen A, Thamsborg SM. Morphological and Genetic Diversity of Trichuris spp. recovered from Humans and Pigs. In  One World, one Health - Parasites in a changing landscape. 2009. p. 109-110

Author

Nissen, Sofie ; Nejsum, Peter ; Christensen, Henrik ; Olsen, Annette ; Thamsborg, Stig Milan. / Morphological and Genetic Diversity of Trichuris spp. recovered from Humans and Pigs.  One World, one Health - Parasites in a changing landscape. 2009. pp. 109-110

Bibtex

@inbook{76eb5fb0a37d11debc73000ea68e967b,
title = "Morphological and Genetic Diversity of Trichuris spp. recovered from Humans and Pigs",
abstract = "The nematodes, Trichuris suis and Trichuris trichiura are believed to be two separate but closely related species. The aim of our study was to examine the morphological and genetic diversity of Trichuris spp. recovered from pigs and humans. Sympatric worm material isolated from 10 humans and 5 pigs in Uganda supplemented with T. suis from Tanzania, Denmark and USA and T. trichiura from England, was obtained. Based on morphology, worms from the two hosts could only be discriminated by the length of the male spicule (t-test, p<0.001). The second internal transcribed spacer (ITS-2) in the r-DNA was amplified by PCR and cloned. Between 1 and 6 clones from 20 worm were sequenced, which resulted in 49 human-derived and 45 pig-derived sequences that could be allocated into as many as 56 different haplotypes. A very large intra-individual variation was found within the human-derived sequences (0.2 – 45.0%) compared to the pig derived sequences (0.2 – 1.4%). This was due to the fact that the human-derived worms consisted of two main ITS-2 sequence types; one of them being unique (69% of the human-derived sequences, consensus sequence 481 nucleotides long) and the other being identical to the sequence type found in pig-derived worms (31% of the human-derived worms, consensus sequence 531 nucleotides long). The results indicated that the nematodes found in pigs belong to a genetically distinct species (T. suis) whereas the nematodes in humans showed considerable genetic variability either related to ancestral polymorphism or more recent cross-breeding between T. trichiura and T. suis.",
author = "Sofie Nissen and Peter Nejsum and Henrik Christensen and Annette Olsen and Thamsborg, {Stig Milan}",
year = "2009",
language = "English",
pages = "109--110",
booktitle = " One World, one Health - Parasites in a changing landscape",
note = "null ; Conference date: 09-08-2009 Through 13-08-2009",

}

RIS

TY - ABST

T1 - Morphological and Genetic Diversity of Trichuris spp. recovered from Humans and Pigs

AU - Nissen, Sofie

AU - Nejsum, Peter

AU - Christensen, Henrik

AU - Olsen, Annette

AU - Thamsborg, Stig Milan

N1 - Conference code: 22

PY - 2009

Y1 - 2009

N2 - The nematodes, Trichuris suis and Trichuris trichiura are believed to be two separate but closely related species. The aim of our study was to examine the morphological and genetic diversity of Trichuris spp. recovered from pigs and humans. Sympatric worm material isolated from 10 humans and 5 pigs in Uganda supplemented with T. suis from Tanzania, Denmark and USA and T. trichiura from England, was obtained. Based on morphology, worms from the two hosts could only be discriminated by the length of the male spicule (t-test, p<0.001). The second internal transcribed spacer (ITS-2) in the r-DNA was amplified by PCR and cloned. Between 1 and 6 clones from 20 worm were sequenced, which resulted in 49 human-derived and 45 pig-derived sequences that could be allocated into as many as 56 different haplotypes. A very large intra-individual variation was found within the human-derived sequences (0.2 – 45.0%) compared to the pig derived sequences (0.2 – 1.4%). This was due to the fact that the human-derived worms consisted of two main ITS-2 sequence types; one of them being unique (69% of the human-derived sequences, consensus sequence 481 nucleotides long) and the other being identical to the sequence type found in pig-derived worms (31% of the human-derived worms, consensus sequence 531 nucleotides long). The results indicated that the nematodes found in pigs belong to a genetically distinct species (T. suis) whereas the nematodes in humans showed considerable genetic variability either related to ancestral polymorphism or more recent cross-breeding between T. trichiura and T. suis.

AB - The nematodes, Trichuris suis and Trichuris trichiura are believed to be two separate but closely related species. The aim of our study was to examine the morphological and genetic diversity of Trichuris spp. recovered from pigs and humans. Sympatric worm material isolated from 10 humans and 5 pigs in Uganda supplemented with T. suis from Tanzania, Denmark and USA and T. trichiura from England, was obtained. Based on morphology, worms from the two hosts could only be discriminated by the length of the male spicule (t-test, p<0.001). The second internal transcribed spacer (ITS-2) in the r-DNA was amplified by PCR and cloned. Between 1 and 6 clones from 20 worm were sequenced, which resulted in 49 human-derived and 45 pig-derived sequences that could be allocated into as many as 56 different haplotypes. A very large intra-individual variation was found within the human-derived sequences (0.2 – 45.0%) compared to the pig derived sequences (0.2 – 1.4%). This was due to the fact that the human-derived worms consisted of two main ITS-2 sequence types; one of them being unique (69% of the human-derived sequences, consensus sequence 481 nucleotides long) and the other being identical to the sequence type found in pig-derived worms (31% of the human-derived worms, consensus sequence 531 nucleotides long). The results indicated that the nematodes found in pigs belong to a genetically distinct species (T. suis) whereas the nematodes in humans showed considerable genetic variability either related to ancestral polymorphism or more recent cross-breeding between T. trichiura and T. suis.

M3 - Conference abstract in proceedings

SP - 109

EP - 110

BT -  One World, one Health - Parasites in a changing landscape

Y2 - 9 August 2009 through 13 August 2009

ER -

ID: 14491233