Microsensor measurements of sulfate reduction and sulfide oxidation in compact microbial communities of aerobic biofilms
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Microsensor measurements of sulfate reduction and sulfide oxidation in compact microbial communities of aerobic biofilms. / Kühl, Michael; Jørgensen, Bo Barker.
In: Applied and Environmental Microbiology, Vol. 58, No. 4, 1992, p. 1164-1174.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Microsensor measurements of sulfate reduction and sulfide oxidation in compact microbial communities of aerobic biofilms
AU - Kühl, Michael
AU - Jørgensen, Bo Barker
PY - 1992
Y1 - 1992
N2 - The microzonation of O2 respiration, H2S oxidation, and SO4 2- reduction in aerobic trickling-filter biofilms was studied by measuring concentration profiles at high spatial resolution (25 to 100 μm) with microsensors for O2, S2-, and pH. Specific reaction rates were calculated from measured concentration profiles by using a simple one-dimensional diffusion reaction model. The importance of electron acceptor and electron donor availability for the microzonation of respiratory processes and their reaction rates was investigated. Oxygen respiration was found in the upper 0.2 to 0.4 mm of the biofilm, whereas sulfate reduction occurred in deeper, anoxic parts of the biofilm. Sulfate reduction accounted for up to 50% of the total mineralization of organic carbon in the biofilms. All H2S produced from sulfate reduction was reoxidized by O2 in a narrow reaction zone, and no H2S escaped to the overlying water. Turnover times of H2S and O2 in the reaction zone were only a few seconds owing to rapid bacterial H2S oxidation. Anaerobic H2S oxidation with NO3 - could be induced by addition of nitrate to the medium. Total sulfate reduction rates increased when the availability of SO4 2- or organic substrate increased as a result of deepening of the sulfate reduction zone or an increase in the sulfate reduction intensity, respectively.
AB - The microzonation of O2 respiration, H2S oxidation, and SO4 2- reduction in aerobic trickling-filter biofilms was studied by measuring concentration profiles at high spatial resolution (25 to 100 μm) with microsensors for O2, S2-, and pH. Specific reaction rates were calculated from measured concentration profiles by using a simple one-dimensional diffusion reaction model. The importance of electron acceptor and electron donor availability for the microzonation of respiratory processes and their reaction rates was investigated. Oxygen respiration was found in the upper 0.2 to 0.4 mm of the biofilm, whereas sulfate reduction occurred in deeper, anoxic parts of the biofilm. Sulfate reduction accounted for up to 50% of the total mineralization of organic carbon in the biofilms. All H2S produced from sulfate reduction was reoxidized by O2 in a narrow reaction zone, and no H2S escaped to the overlying water. Turnover times of H2S and O2 in the reaction zone were only a few seconds owing to rapid bacterial H2S oxidation. Anaerobic H2S oxidation with NO3 - could be induced by addition of nitrate to the medium. Total sulfate reduction rates increased when the availability of SO4 2- or organic substrate increased as a result of deepening of the sulfate reduction zone or an increase in the sulfate reduction intensity, respectively.
U2 - 10.1128/aem.58.4.1164-1174.1992
DO - 10.1128/aem.58.4.1164-1174.1992
M3 - Journal article
AN - SCOPUS:0026581545
VL - 58
SP - 1164
EP - 1174
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 4
ER -
ID: 201685365