Methodological approaches to the study of metabolism across individual tissues in man
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Methodological approaches to the study of metabolism across individual tissues in man. / Magkos, Faidon; Sidossis, Labros S.
In: Current Opinion in Clinical Nutrition and Metabolic Care, Vol. 8, No. 5, 2005, p. 501-510.Research output: Contribution to journal › Review › Research › peer-review
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TY - JOUR
T1 - Methodological approaches to the study of metabolism across individual tissues in man
AU - Magkos, Faidon
AU - Sidossis, Labros S
N1 - (Ekstern)
PY - 2005
Y1 - 2005
N2 - Purpose of review: This article is intended to briefly overview available methodological approaches for the study of regional metabolism in man in vivo, and to summarize recent advances in this field of research.Recent findings: Several methods have been developed and currently allow for the qualitative and quantitative assessment of energy interconversions and substrate fluxes across individual tissues of man, including the measurement of arteriovenous concentration differences, microdialysis, and nuclear magnetic resonance spectroscopy of carbon, hydrogen, and phosphorus isotopes. Each method alone has been used rather extensively to examine certain aspects of organ and tissue metabolism under a variety of experimental conditions, and has contributed novel information in this regard. The most exciting development appears to be the combined use of more than one investigational technique, across one or more tissues simultaneously. A handful of recent studies have employed complex experimental designs or hybrid methodologies, ultimately demonstrating the potential for a more detailed assessment of metabolism at the local level.Summary: Clearly, advances in the use, performance, and applications of available methods are expected to provide improved and more powerful tools for the metabolic investigation of organs and tissues in humans in vivo.
AB - Purpose of review: This article is intended to briefly overview available methodological approaches for the study of regional metabolism in man in vivo, and to summarize recent advances in this field of research.Recent findings: Several methods have been developed and currently allow for the qualitative and quantitative assessment of energy interconversions and substrate fluxes across individual tissues of man, including the measurement of arteriovenous concentration differences, microdialysis, and nuclear magnetic resonance spectroscopy of carbon, hydrogen, and phosphorus isotopes. Each method alone has been used rather extensively to examine certain aspects of organ and tissue metabolism under a variety of experimental conditions, and has contributed novel information in this regard. The most exciting development appears to be the combined use of more than one investigational technique, across one or more tissues simultaneously. A handful of recent studies have employed complex experimental designs or hybrid methodologies, ultimately demonstrating the potential for a more detailed assessment of metabolism at the local level.Summary: Clearly, advances in the use, performance, and applications of available methods are expected to provide improved and more powerful tools for the metabolic investigation of organs and tissues in humans in vivo.
KW - Blood Flow Velocity
KW - Carbon Isotopes
KW - Energy Metabolism/physiology
KW - Humans
KW - Isotope Labeling/methods
KW - Magnetic Resonance Spectroscopy/methods
KW - Microdialysis/methods
KW - Phosphorus Isotopes
U2 - 10.1097/01.mco.0000170756.71114.78
DO - 10.1097/01.mco.0000170756.71114.78
M3 - Review
C2 - 16079620
VL - 8
SP - 501
EP - 510
JO - Current Opinion in Clinical Nutrition and Metabolic Care
JF - Current Opinion in Clinical Nutrition and Metabolic Care
SN - 1363-1950
IS - 5
ER -
ID: 297232212