Lymph Nodes Draining Infections Investigated by PET and Immunohistochemistry in a Juvenile Porcine Model
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Lymph Nodes Draining Infections Investigated by PET and Immunohistochemistry in a Juvenile Porcine Model. / Afzelius, Pia; Morsing, Malene Kjelin; Nielsen, Ole Lerberg; Alstrup, Aage Kristian Olsen; Jensen, Svend Borup; Jødal, Lars.
In: Molecules, Vol. 27, No. 9, 2792, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Lymph Nodes Draining Infections Investigated by PET and Immunohistochemistry in a Juvenile Porcine Model
AU - Afzelius, Pia
AU - Morsing, Malene Kjelin
AU - Nielsen, Ole Lerberg
AU - Alstrup, Aage Kristian Olsen
AU - Jensen, Svend Borup
AU - Jødal, Lars
N1 - Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022
Y1 - 2022
N2 - Background: [18 F]FDG and [11 C]methionine accumulate in lymph nodes draining S. aureus-infected foci. The lymph nodes were characterized by weight, [11 C]methionine-and [18 F]FDG-positron emissions tomography (PET)/computed tomography (CT), and immunohistochemical (IHC)-staining. Methods: 20 pigs inoculated with S. aureus into the right femoral artery were PET/CT-scanned with [18 F]FDG, and nine of the pigs were additionally scanned with [11 C]methionine. Mammary, medial iliac, and popliteal lymph nodes from the left and right hind limbs were weighed. IHC-staining for calculations of area fractions of Ki-67, L1, and IL-8 positive cells was done in mammary and popliteal lymph nodes from the nine pigs. Results: The pigs developed one to six osteomyelitis foci. Some pigs developed contiguous infections of peri-osseous tissue and inoculationsite abscesses. Weights of mammary and medial iliac lymph nodes and their [18 F]FDG maximum Standardized Uptake Values (SUVFDGmax) showed a significant increase in the inoculated limb compared to the left limb. Popliteal lymph node weight and their FDG uptake did not differ significantly between hind limbs. Area fractions of Ki-67 and IL-8 in the right mammary lymph nodes and SUVMetmax in the right popliteal lymph nodes were significantly increased compared with the left side. Conclusion: The PET-tracers [18 F]FDG and [11 C]methionine, and the IHC-markers Ki-67 and IL-8, but not L1, showed increased values in lymph nodes draining soft tissues infected with S. aureus. The increase in [11 C]methionine may indicate a more acute lymph node response, whereas an increase in [18 F]FDG may indicate a more chronic response.
AB - Background: [18 F]FDG and [11 C]methionine accumulate in lymph nodes draining S. aureus-infected foci. The lymph nodes were characterized by weight, [11 C]methionine-and [18 F]FDG-positron emissions tomography (PET)/computed tomography (CT), and immunohistochemical (IHC)-staining. Methods: 20 pigs inoculated with S. aureus into the right femoral artery were PET/CT-scanned with [18 F]FDG, and nine of the pigs were additionally scanned with [11 C]methionine. Mammary, medial iliac, and popliteal lymph nodes from the left and right hind limbs were weighed. IHC-staining for calculations of area fractions of Ki-67, L1, and IL-8 positive cells was done in mammary and popliteal lymph nodes from the nine pigs. Results: The pigs developed one to six osteomyelitis foci. Some pigs developed contiguous infections of peri-osseous tissue and inoculationsite abscesses. Weights of mammary and medial iliac lymph nodes and their [18 F]FDG maximum Standardized Uptake Values (SUVFDGmax) showed a significant increase in the inoculated limb compared to the left limb. Popliteal lymph node weight and their FDG uptake did not differ significantly between hind limbs. Area fractions of Ki-67 and IL-8 in the right mammary lymph nodes and SUVMetmax in the right popliteal lymph nodes were significantly increased compared with the left side. Conclusion: The PET-tracers [18 F]FDG and [11 C]methionine, and the IHC-markers Ki-67 and IL-8, but not L1, showed increased values in lymph nodes draining soft tissues infected with S. aureus. The increase in [11 C]methionine may indicate a more acute lymph node response, whereas an increase in [18 F]FDG may indicate a more chronic response.
KW - calcium-binding leukocyte L1
KW - IL-8
KW - immunohistochemistry
KW - Ki-67
KW - lymph nodes
KW - PET
KW - pig model
KW - Staphylococcus aureus
KW - [ C]methionine
KW - [ F]FDG
U2 - 10.3390/molecules27092792
DO - 10.3390/molecules27092792
M3 - Journal article
C2 - 35566137
AN - SCOPUS:85129831693
VL - 27
JO - Molecules
JF - Molecules
SN - 1420-3049
IS - 9
M1 - 2792
ER -
ID: 308121925