Lignin from hydrothermally pretreated grass biomass retards enzymatic cellulose degradation by acting as a physical barrier rather than by inducing nonproductive adsorption of enzymes
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Lignin from hydrothermally pretreated grass biomass retards enzymatic cellulose degradation by acting as a physical barrier rather than by inducing nonproductive adsorption of enzymes. / Djajadi, Demi T.; Jensen, Mads M. ; Oliveira, Marlene; Jensen, Anders; Thygesen, Lisbeth Garbrecht; Pinelo, Manuel; Glasius, Marianne; Jørgensen, Henning; Meyer, Anne S.
In: Biotechnology for Biofuels, Vol. 11, No. 1, 85, 2018.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Lignin from hydrothermally pretreated grass biomass retards enzymatic cellulose degradation by acting as a physical barrier rather than by inducing nonproductive adsorption of enzymes
AU - Djajadi, Demi T.
AU - Jensen, Mads M.
AU - Oliveira, Marlene
AU - Jensen, Anders
AU - Thygesen, Lisbeth Garbrecht
AU - Pinelo, Manuel
AU - Glasius, Marianne
AU - Jørgensen, Henning
AU - Meyer, Anne S.
PY - 2018
Y1 - 2018
N2 - Background: Lignin is known to hinder efficient enzymatic conversion of lignocellulose in biorefining processes. In particular, nonproductive adsorption of cellulases onto lignin is considered a key mechanism to explain how lignin retards enzymatic cellulose conversion in extended reactions. Results: Lignin-rich residues (LRRs) were prepared via extensive enzymatic cellulose degradation of corn stover (Zea mays subsp. mays L.), Miscanthus × giganteus stalks (MS) and wheat straw (Triticum aestivum L.) (WS) samples that each had been hydrothermally pretreated at three severity factors (log R 0) of 3.65, 3.83 and 3.97. The LRRs had different residual carbohydrate levels - the highest in MS; the lowest in WS. The residual carbohydrate was not traceable at the surface of the LRRs particles by ATR-FTIR analysis. The chemical properties of the lignin in the LRRs varied across the three types of biomass, but monolignols composition was not affected by the severity factor. When pure cellulose was added to a mixture of LRRs and a commercial cellulolytic enzyme preparation, the rate and extent of glucose release were unaffected by the presence of LRRs regardless of biomass type and severity factor, despite adsorption of the enzymes to the LRRs. Since the surface of the LRRs particles were covered by lignin, the data suggest that the retardation of enzymatic cellulose degradation during extended reaction on lignocellulosic substrates is due to physical blockage of the access of enzymes to the cellulose caused by the gradual accumulation of lignin at the surface of the biomass particles rather than by nonproductive enzyme adsorption. Conclusions: The study suggests that lignin from hydrothermally pretreated grass biomass retards enzymatic cellulose degradation by acting as a physical barrier blocking the access of enzymes to cellulose rather than by inducing retardation through nonproductive adsorption of enzymes.
AB - Background: Lignin is known to hinder efficient enzymatic conversion of lignocellulose in biorefining processes. In particular, nonproductive adsorption of cellulases onto lignin is considered a key mechanism to explain how lignin retards enzymatic cellulose conversion in extended reactions. Results: Lignin-rich residues (LRRs) were prepared via extensive enzymatic cellulose degradation of corn stover (Zea mays subsp. mays L.), Miscanthus × giganteus stalks (MS) and wheat straw (Triticum aestivum L.) (WS) samples that each had been hydrothermally pretreated at three severity factors (log R 0) of 3.65, 3.83 and 3.97. The LRRs had different residual carbohydrate levels - the highest in MS; the lowest in WS. The residual carbohydrate was not traceable at the surface of the LRRs particles by ATR-FTIR analysis. The chemical properties of the lignin in the LRRs varied across the three types of biomass, but monolignols composition was not affected by the severity factor. When pure cellulose was added to a mixture of LRRs and a commercial cellulolytic enzyme preparation, the rate and extent of glucose release were unaffected by the presence of LRRs regardless of biomass type and severity factor, despite adsorption of the enzymes to the LRRs. Since the surface of the LRRs particles were covered by lignin, the data suggest that the retardation of enzymatic cellulose degradation during extended reaction on lignocellulosic substrates is due to physical blockage of the access of enzymes to the cellulose caused by the gradual accumulation of lignin at the surface of the biomass particles rather than by nonproductive enzyme adsorption. Conclusions: The study suggests that lignin from hydrothermally pretreated grass biomass retards enzymatic cellulose degradation by acting as a physical barrier blocking the access of enzymes to cellulose rather than by inducing retardation through nonproductive adsorption of enzymes.
KW - Adsorption
KW - Apparent surface abundance
KW - Cellulases
KW - Depolymerization
KW - Enzymatic hydrolysis
KW - Inhibition
KW - Lignin
KW - Physical barrier
KW - S/G ratio
KW - β-O-4 linkage
U2 - 10.1186/s13068-018-1085-0
DO - 10.1186/s13068-018-1085-0
M3 - Journal article
C2 - 29619081
AN - SCOPUS:85044728782
VL - 11
JO - Biotechnology for Biofuels
JF - Biotechnology for Biofuels
SN - 1754-6834
IS - 1
M1 - 85
ER -
ID: 195296209