Isolation and elucidation of antibacterial compounds from roots and stems of Synadenium glaucescens Pax
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Isolation and elucidation of antibacterial compounds from roots and stems of Synadenium glaucescens Pax. / Rwegoshora, F.; Msengwa, Z.; Mabiki, F.; Machumi, F.; Chacha, M.; Styrishave, B.; Cornett, C.
In: East African Journal of Science, Technology and Innovation, Vol. 4, No. 2, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Isolation and elucidation of antibacterial compounds from roots and stems of Synadenium glaucescens Pax
AU - Rwegoshora, F.
AU - Msengwa, Z.
AU - Mabiki, F.
AU - Machumi, F.
AU - Chacha, M.
AU - Styrishave, B.
AU - Cornett, C.
N1 - Publisher Copyright: © 2023, East African Science and Technology Commission - EASTECO KG. All rights reserved.
PY - 2023
Y1 - 2023
N2 - Extracts of Synadenium glaucescens Pax are reported to contain biologically active compounds against bacterial and viral infections. This study aimed at isolating pure compounds from its bioactive extracts as well as evaluating their antibacterial efficacy. A phytochemical investigation of the root and stem barks involved total maceration in methanol (MeOH). The root barks extract was then partitioned by Vacuum Liquid Chromatography (VLC) in a solvent gradient system to afford three fractions of Hexane (Hex), Ethyl acetate (EtOAc) and finally MeOH for the least, moderate, and most polar compounds respectively. The MeOH extract of stem barks was also partitioned in the same way by using two solvents: dichloromethane (DCM) and MeOH. All fractions were finally dried on a rotary evaporator at < 60 OC of the water bath. Isolation of pure compound from the EtOAc and DCM led to isolation of four compounds namely, hexacosane (G1), β-sitosterol (G2), octacosyl ferulate (G3) and hexacosanoic acid (G4). Their structures were analyzed and confirmed through NMR, GC-MS and in comparison, with literature. Antibacterial assay for G1, G3 and G4 against P. aeruginosa, E. coli, S. aureus, and E. faecalis was achieved by broth serial microdilution. Compounds, G3 demonstrated strong activity against S. aureus (MIC = 0.125 mg/mL) and weak activity against the rest strains (MIC = 2 mg/ mL). Also, the test results indicated G1 had weak activity against all tested strains (MIC = 2 mg/mL or above). While G4 demonstrated a moderate activity (1.0 mg/mL) against E. coli, S. aureus, E. faecalis and weak against P. aeruginosa (MIC > 2 mg/mL). These findings support traditional use and promise for antibacterial drug agents from S. glaucescens Pax.
AB - Extracts of Synadenium glaucescens Pax are reported to contain biologically active compounds against bacterial and viral infections. This study aimed at isolating pure compounds from its bioactive extracts as well as evaluating their antibacterial efficacy. A phytochemical investigation of the root and stem barks involved total maceration in methanol (MeOH). The root barks extract was then partitioned by Vacuum Liquid Chromatography (VLC) in a solvent gradient system to afford three fractions of Hexane (Hex), Ethyl acetate (EtOAc) and finally MeOH for the least, moderate, and most polar compounds respectively. The MeOH extract of stem barks was also partitioned in the same way by using two solvents: dichloromethane (DCM) and MeOH. All fractions were finally dried on a rotary evaporator at < 60 OC of the water bath. Isolation of pure compound from the EtOAc and DCM led to isolation of four compounds namely, hexacosane (G1), β-sitosterol (G2), octacosyl ferulate (G3) and hexacosanoic acid (G4). Their structures were analyzed and confirmed through NMR, GC-MS and in comparison, with literature. Antibacterial assay for G1, G3 and G4 against P. aeruginosa, E. coli, S. aureus, and E. faecalis was achieved by broth serial microdilution. Compounds, G3 demonstrated strong activity against S. aureus (MIC = 0.125 mg/mL) and weak activity against the rest strains (MIC = 2 mg/ mL). Also, the test results indicated G1 had weak activity against all tested strains (MIC = 2 mg/mL or above). While G4 demonstrated a moderate activity (1.0 mg/mL) against E. coli, S. aureus, E. faecalis and weak against P. aeruginosa (MIC > 2 mg/mL). These findings support traditional use and promise for antibacterial drug agents from S. glaucescens Pax.
KW - antimicrobial
KW - drug
KW - Euphorbiaceae
KW - ferulic acid
KW - NMR
KW - octacosylferulate
KW - phytochemistry
U2 - 10.37425/eajsti.v4i3.669
DO - 10.37425/eajsti.v4i3.669
M3 - Journal article
AN - SCOPUS:85181942874
VL - 4
JO - East African Journal of Science, Technology and Innovation
JF - East African Journal of Science, Technology and Innovation
SN - 2707-0425
IS - 2
ER -
ID: 391675790