In vitro bioassay investigations of the endocrine disrupting potential of steviol glycosides and their metabolite steviol, components of the natural sweetener Stevia

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

In vitro bioassay investigations of the endocrine disrupting potential of steviol glycosides and their metabolite steviol, components of the natural sweetener Stevia. / Shannon, Maeve; Rehfeld, Anders; Frizzell, Caroline; Livingstone, Christina; McGonagle, Caoimhe; Skakkebaek, Niels E; Wielogórska, Ewa; Connolly, Lisa.

In: Molecular and Cellular Endocrinology, Vol. 427, 15.05.2016, p. 65-72.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Shannon, M, Rehfeld, A, Frizzell, C, Livingstone, C, McGonagle, C, Skakkebaek, NE, Wielogórska, E & Connolly, L 2016, 'In vitro bioassay investigations of the endocrine disrupting potential of steviol glycosides and their metabolite steviol, components of the natural sweetener Stevia', Molecular and Cellular Endocrinology, vol. 427, pp. 65-72. https://doi.org/10.1016/j.mce.2016.03.005

APA

Shannon, M., Rehfeld, A., Frizzell, C., Livingstone, C., McGonagle, C., Skakkebaek, N. E., Wielogórska, E., & Connolly, L. (2016). In vitro bioassay investigations of the endocrine disrupting potential of steviol glycosides and their metabolite steviol, components of the natural sweetener Stevia. Molecular and Cellular Endocrinology, 427, 65-72. https://doi.org/10.1016/j.mce.2016.03.005

Vancouver

Shannon M, Rehfeld A, Frizzell C, Livingstone C, McGonagle C, Skakkebaek NE et al. In vitro bioassay investigations of the endocrine disrupting potential of steviol glycosides and their metabolite steviol, components of the natural sweetener Stevia. Molecular and Cellular Endocrinology. 2016 May 15;427:65-72. https://doi.org/10.1016/j.mce.2016.03.005

Author

Shannon, Maeve ; Rehfeld, Anders ; Frizzell, Caroline ; Livingstone, Christina ; McGonagle, Caoimhe ; Skakkebaek, Niels E ; Wielogórska, Ewa ; Connolly, Lisa. / In vitro bioassay investigations of the endocrine disrupting potential of steviol glycosides and their metabolite steviol, components of the natural sweetener Stevia. In: Molecular and Cellular Endocrinology. 2016 ; Vol. 427. pp. 65-72.

Bibtex

@article{9eb3df56f75147fb9e3da1eada6ca2e4,

title = "In vitro bioassay investigations of the endocrine disrupting potential of steviol glycosides and their metabolite steviol, components of the natural sweetener Stevia",

abstract = "The food industry is moving towards the use of natural sweeteners such as those produced by Stevia rebaudiana due to the number of health and safety concerns surrounding artificial sweeteners. Despite the fact that these sweeteners are natural; they cannot be assumed safe. Steviol glycosides have a steroidal structure and therefore may have the potential to act as an endocrine disruptor in the body. Reporter gene assays (RGAs), H295R steroidogenesis assay and Ca(2+) fluorimetry based assays using human sperm cells have been used to assess the endocrine disrupting potential of two steviol glycosides: stevioside and rebaudioside A, and their metabolite steviol. A decrease in transcriptional activity of the progestagen receptor was seen following treatment with 25,000 ng/ml steviol in the presence of progesterone (157 ng/ml) resulting in a 31% decrease in progestagen response (p=<0.01). At the level of steroidogenesis, the metabolite steviol (500-25,000 ng/ml) increased progesterone production significantly by 2.3 fold when exposed to 10,000 ng/ml (p=<0.05) and 5 fold when exposed to 25,000 ng/ml (p=<0.001). Additionally, steviol was found to induce an agonistic response on CatSper, a progesterone receptor of sperm, causing a rapid influx of Ca(2+). The response was fully inhibited using a specific CatSper inhibitor. These findings highlight the potential for steviol to act as a potential endocrine disruptor.",

author = "Maeve Shannon and Anders Rehfeld and Caroline Frizzell and Christina Livingstone and Caoimhe McGonagle and Skakkebaek, {Niels E} and Ewa Wielog{\'o}rska and Lisa Connolly",

year = "2016",

month = may,

day = "15",

doi = "10.1016/j.mce.2016.03.005",

language = "English",

volume = "427",

pages = "65--72",

journal = "Molecular and Cellular Endocrinology",

issn = "0303-7207",

publisher = "Elsevier Ireland Ltd",

}

RIS

TY - JOUR

T1 - In vitro bioassay investigations of the endocrine disrupting potential of steviol glycosides and their metabolite steviol, components of the natural sweetener Stevia

AU - Shannon, Maeve

AU - Rehfeld, Anders

AU - Frizzell, Caroline

AU - Livingstone, Christina

AU - McGonagle, Caoimhe

AU - Skakkebaek, Niels E

AU - Wielogórska, Ewa

AU - Connolly, Lisa

PY - 2016/5/15

Y1 - 2016/5/15

N2 - The food industry is moving towards the use of natural sweeteners such as those produced by Stevia rebaudiana due to the number of health and safety concerns surrounding artificial sweeteners. Despite the fact that these sweeteners are natural; they cannot be assumed safe. Steviol glycosides have a steroidal structure and therefore may have the potential to act as an endocrine disruptor in the body. Reporter gene assays (RGAs), H295R steroidogenesis assay and Ca(2+) fluorimetry based assays using human sperm cells have been used to assess the endocrine disrupting potential of two steviol glycosides: stevioside and rebaudioside A, and their metabolite steviol. A decrease in transcriptional activity of the progestagen receptor was seen following treatment with 25,000 ng/ml steviol in the presence of progesterone (157 ng/ml) resulting in a 31% decrease in progestagen response (p=<0.01). At the level of steroidogenesis, the metabolite steviol (500-25,000 ng/ml) increased progesterone production significantly by 2.3 fold when exposed to 10,000 ng/ml (p=<0.05) and 5 fold when exposed to 25,000 ng/ml (p=<0.001). Additionally, steviol was found to induce an agonistic response on CatSper, a progesterone receptor of sperm, causing a rapid influx of Ca(2+). The response was fully inhibited using a specific CatSper inhibitor. These findings highlight the potential for steviol to act as a potential endocrine disruptor.

AB - The food industry is moving towards the use of natural sweeteners such as those produced by Stevia rebaudiana due to the number of health and safety concerns surrounding artificial sweeteners. Despite the fact that these sweeteners are natural; they cannot be assumed safe. Steviol glycosides have a steroidal structure and therefore may have the potential to act as an endocrine disruptor in the body. Reporter gene assays (RGAs), H295R steroidogenesis assay and Ca(2+) fluorimetry based assays using human sperm cells have been used to assess the endocrine disrupting potential of two steviol glycosides: stevioside and rebaudioside A, and their metabolite steviol. A decrease in transcriptional activity of the progestagen receptor was seen following treatment with 25,000 ng/ml steviol in the presence of progesterone (157 ng/ml) resulting in a 31% decrease in progestagen response (p=<0.01). At the level of steroidogenesis, the metabolite steviol (500-25,000 ng/ml) increased progesterone production significantly by 2.3 fold when exposed to 10,000 ng/ml (p=<0.05) and 5 fold when exposed to 25,000 ng/ml (p=<0.001). Additionally, steviol was found to induce an agonistic response on CatSper, a progesterone receptor of sperm, causing a rapid influx of Ca(2+). The response was fully inhibited using a specific CatSper inhibitor. These findings highlight the potential for steviol to act as a potential endocrine disruptor.

U2 - 10.1016/j.mce.2016.03.005

DO - 10.1016/j.mce.2016.03.005

M3 - Journal article

C2 - 26965840

VL - 427

SP - 65

EP - 72

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

SN - 0303-7207

ER -

ID: 160107397