Insulin action in human thighs after one-legged immobilization
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Insulin action in human thighs after one-legged immobilization. / Richter, Erik A.; Kiens, Bente; Mizuno, M.; Strange, Søren.
In: Journal of Applied Physiology, Vol. 67, No. 1, 1989, p. 19-23.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Insulin action in human thighs after one-legged immobilization
AU - Richter, Erik A.
AU - Kiens, Bente
AU - Mizuno, M.
AU - Strange, Søren
PY - 1989
Y1 - 1989
N2 - Insulin action was assessed in thighs of five healthy young males who had one knee immobilized for 7 days by a splint. The splint was not worn in bed. Subjects also used crutches to prevent weight bearing of the immobilized leg. Immobilization decreased the activity of citrate synthase and 3-OH-acyl-CoA-dehydrogenase in the vastus lateralis muscle by 9 and 14%, respectively, and thigh volume by 5%. After 7 days of immobilization, a two-step euglycemic hyperinsulinemic clamp procedure combined with arterial and bilateral femoral venous catheterization was performed. Insulin action on glucose uptake and tyrosine release of the thighs at mean plasma insulin concentrations of 67 (clamp step I) and 447 microU/ml (clamp step II) was decreased by immobilization, whereas immobilization did not affect insulin action on thigh exchange of free fatty acids, glycerol, O2, or potassium. Before and during the clamp step I, lactate release was significantly higher in the immobilized than in the control thigh. Seven days of one-legged immobilization causes local decreased insulin action on thigh glucose uptake and net protein degradation.
AB - Insulin action was assessed in thighs of five healthy young males who had one knee immobilized for 7 days by a splint. The splint was not worn in bed. Subjects also used crutches to prevent weight bearing of the immobilized leg. Immobilization decreased the activity of citrate synthase and 3-OH-acyl-CoA-dehydrogenase in the vastus lateralis muscle by 9 and 14%, respectively, and thigh volume by 5%. After 7 days of immobilization, a two-step euglycemic hyperinsulinemic clamp procedure combined with arterial and bilateral femoral venous catheterization was performed. Insulin action on glucose uptake and tyrosine release of the thighs at mean plasma insulin concentrations of 67 (clamp step I) and 447 microU/ml (clamp step II) was decreased by immobilization, whereas immobilization did not affect insulin action on thigh exchange of free fatty acids, glycerol, O2, or potassium. Before and during the clamp step I, lactate release was significantly higher in the immobilized than in the control thigh. Seven days of one-legged immobilization causes local decreased insulin action on thigh glucose uptake and net protein degradation.
KW - Adult
KW - Glucose
KW - Humans
KW - Immobilization
KW - Insulin
KW - Insulin Resistance
KW - Male
KW - Muscle Proteins
KW - Muscles
KW - Thigh
M3 - Journal article
C2 - 2668254
VL - 67
SP - 19
EP - 23
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology
SN - 8750-7587
IS - 1
ER -
ID: 154756678