Inhibition of yeast growth during long term exposure to laser light around 1064 nm
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Inhibition of yeast growth during long term exposure to laser light around 1064 nm. / Aabo, Thomas; Perch-Nielsen, Ivan R.; Dam, Jeppe Seidelin; Palima, Darwin Z.; Siegumfeldt, Henrik; Glückstad, Jesper; Arneborg, Nils.
In: Proceedings of SPIE, the International Society for Optical Engineering, 2009.Research output: Contribution to journal › Conference article › Research › peer-review
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TY - GEN
T1 - Inhibition of yeast growth during long term exposure to laser light around 1064 nm
AU - Aabo, Thomas
AU - Perch-Nielsen, Ivan R.
AU - Dam, Jeppe Seidelin
AU - Palima, Darwin Z.
AU - Siegumfeldt, Henrik
AU - Glückstad, Jesper
AU - Arneborg, Nils
N1 - Conference code: 3
PY - 2009
Y1 - 2009
N2 - We have studied the effect of a 1070 nm continuous wave Ytterbium fiber laser on exponentially growing Saccharomyces cerevisiae yeast cells over a span of 4 hours. The cells were immobilized onto Concanavalin A covered yeast cells over a span of 4 hours. The cells were immobilized onto Concanavalin A covered microscope slides and the growth was measured using the area increase of the cells in 2D. Using a continuous dual beam plane wave with a uniform spatial intensity distribution, we found that a continuous radiant flux through a single cell as low as 0.5 mW in 1.5 hours significantly changed the growth and division rate of S. cerevisiae. With the dual beam setup used we were able to successfully manipulate single S. cerevisiae cells in 3 dimensions with a minimum flux thorough the cell of 3.5 mW. In the regime investigated from 0.7 mW to 2.6 mW we found no threshold for the photo damage, but rather a continuous response to the increased accumulated dose.
AB - We have studied the effect of a 1070 nm continuous wave Ytterbium fiber laser on exponentially growing Saccharomyces cerevisiae yeast cells over a span of 4 hours. The cells were immobilized onto Concanavalin A covered yeast cells over a span of 4 hours. The cells were immobilized onto Concanavalin A covered microscope slides and the growth was measured using the area increase of the cells in 2D. Using a continuous dual beam plane wave with a uniform spatial intensity distribution, we found that a continuous radiant flux through a single cell as low as 0.5 mW in 1.5 hours significantly changed the growth and division rate of S. cerevisiae. With the dual beam setup used we were able to successfully manipulate single S. cerevisiae cells in 3 dimensions with a minimum flux thorough the cell of 3.5 mW. In the regime investigated from 0.7 mW to 2.6 mW we found no threshold for the photo damage, but rather a continuous response to the increased accumulated dose.
U2 - 10.1117/12.810146
DO - 10.1117/12.810146
M3 - Conference article
JO - Progress in Biomedical Optics and Imaging
JF - Progress in Biomedical Optics and Imaging
SN - 1605-7422
Y2 - 28 January 2009
ER -
ID: 18651875