Identifying protective Streptococcus pyogenes vaccine antigens recognized by both B and T cells in human adults and children

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Identifying protective Streptococcus pyogenes vaccine antigens recognized by both B and T cells in human adults and children. / Mortensen, Rasmus; Nissen, Thomas Nørrelykke; Fredslund, Sine; Rosenkrands, Ida; Christensen, Jan Pravsgaard; Andersen, Peter; Dietrich, Jes.

In: Scientific Reports, Vol. 6, 22030, 2016.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Mortensen, R, Nissen, TN, Fredslund, S, Rosenkrands, I, Christensen, JP, Andersen, P & Dietrich, J 2016, 'Identifying protective Streptococcus pyogenes vaccine antigens recognized by both B and T cells in human adults and children', Scientific Reports, vol. 6, 22030. https://doi.org/10.1038/srep22030

APA

Mortensen, R., Nissen, T. N., Fredslund, S., Rosenkrands, I., Christensen, J. P., Andersen, P., & Dietrich, J. (2016). Identifying protective Streptococcus pyogenes vaccine antigens recognized by both B and T cells in human adults and children. Scientific Reports, 6, [22030]. https://doi.org/10.1038/srep22030

Vancouver

Mortensen R, Nissen TN, Fredslund S, Rosenkrands I, Christensen JP, Andersen P et al. Identifying protective Streptococcus pyogenes vaccine antigens recognized by both B and T cells in human adults and children. Scientific Reports. 2016;6. 22030. https://doi.org/10.1038/srep22030

Author

Mortensen, Rasmus ; Nissen, Thomas Nørrelykke ; Fredslund, Sine ; Rosenkrands, Ida ; Christensen, Jan Pravsgaard ; Andersen, Peter ; Dietrich, Jes. / Identifying protective Streptococcus pyogenes vaccine antigens recognized by both B and T cells in human adults and children. In: Scientific Reports. 2016 ; Vol. 6.

Bibtex

@article{c9c3c3ae1fc840bf9657e9724c4a63d0,
title = "Identifying protective Streptococcus pyogenes vaccine antigens recognized by both B and T cells in human adults and children",
abstract = "No commercial vaccine exists against Group A streptococci (GAS; Streptococcus pyogenes) and only little is known about anti-GAS protective immunity. In our effort to discover new protective vaccine candidates, we selected 21 antigens based on an in silico evaluation. These were all well-conserved among different GAS strains, upregulated in host-pathogen interaction studies, and predicted to be extracellular or associated with the surface of the bacteria. The antigens were tested for both antibody recognition and T cell responses in human adults and children. The antigenicity of a selected group of antigens was further validated using a high-density peptide array technology that also identified the linear epitopes. Based on immunological recognition, four targets were selected and tested for protective capabilities in an experimental GAS infection model in mice. Shown for the first time, three of these targets (spy0469, spy1228 and spy1801) conferred significant protection whereas one (spy1643) did not.",
author = "Rasmus Mortensen and Nissen, {Thomas N{\o}rrelykke} and Sine Fredslund and Ida Rosenkrands and Christensen, {Jan Pravsgaard} and Peter Andersen and Jes Dietrich",
year = "2016",
doi = "10.1038/srep22030",
language = "English",
volume = "6",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Identifying protective Streptococcus pyogenes vaccine antigens recognized by both B and T cells in human adults and children

AU - Mortensen, Rasmus

AU - Nissen, Thomas Nørrelykke

AU - Fredslund, Sine

AU - Rosenkrands, Ida

AU - Christensen, Jan Pravsgaard

AU - Andersen, Peter

AU - Dietrich, Jes

PY - 2016

Y1 - 2016

N2 - No commercial vaccine exists against Group A streptococci (GAS; Streptococcus pyogenes) and only little is known about anti-GAS protective immunity. In our effort to discover new protective vaccine candidates, we selected 21 antigens based on an in silico evaluation. These were all well-conserved among different GAS strains, upregulated in host-pathogen interaction studies, and predicted to be extracellular or associated with the surface of the bacteria. The antigens were tested for both antibody recognition and T cell responses in human adults and children. The antigenicity of a selected group of antigens was further validated using a high-density peptide array technology that also identified the linear epitopes. Based on immunological recognition, four targets were selected and tested for protective capabilities in an experimental GAS infection model in mice. Shown for the first time, three of these targets (spy0469, spy1228 and spy1801) conferred significant protection whereas one (spy1643) did not.

AB - No commercial vaccine exists against Group A streptococci (GAS; Streptococcus pyogenes) and only little is known about anti-GAS protective immunity. In our effort to discover new protective vaccine candidates, we selected 21 antigens based on an in silico evaluation. These were all well-conserved among different GAS strains, upregulated in host-pathogen interaction studies, and predicted to be extracellular or associated with the surface of the bacteria. The antigens were tested for both antibody recognition and T cell responses in human adults and children. The antigenicity of a selected group of antigens was further validated using a high-density peptide array technology that also identified the linear epitopes. Based on immunological recognition, four targets were selected and tested for protective capabilities in an experimental GAS infection model in mice. Shown for the first time, three of these targets (spy0469, spy1228 and spy1801) conferred significant protection whereas one (spy1643) did not.

U2 - 10.1038/srep22030

DO - 10.1038/srep22030

M3 - Journal article

C2 - 26911649

VL - 6

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 22030

ER -

ID: 163776903