Identification of cross-reacting T-cell epitopes in structural and non-structural proteins of swine and pandemic H1N1 influenza a virus strains in pigs
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Heterologous protection against swine influenza viruses (SwIVs) of different lineages is an important concern for the pig industry. Cross-protection between ‘avian-like’ H1N1 and 2009 pandemic H1N1 lineages has been observed previously, indicating the involvement of cross-reacting T-cells. Here, reverse vaccinology was applied to identify cross-reacting MHC class I T-cell epitopes from two different SwIV H1 lineages in pigs. In silico prediction followed by in vitro and in vivo testing was used to identify SLA-1*0702 T-cell epitopes in heterologous SwIV-infected pigs. Following viral infection, tetramer specific T-cell populations were identified. The majority of the identified T-cell epitopes were conserved between the examined lineages, suggesting that targeting cross-reactive T-cell epitopes could be used to improve vaccines against SwIV in SLA-1*0702-positive pigs.
Original language | English |
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Article number | 000748 |
Journal | Journal of General Virology |
Volume | 98 |
Issue number | 5 |
Pages (from-to) | 895-899 |
Number of pages | 5 |
ISSN | 0022-1317 |
DOIs | |
Publication status | Published - 2017 |
Externally published | Yes |
- Cross-reactive epitopes, MHC class I T cell epitopes, Reverse vaccinology, Swine influenza virus
Research areas
ID: 247394601