Histone H4 lysine 20 mono-methylation directly facilitates chromatin openness and promotes transcription of housekeeping genes

Research output: Contribution to journal › Journal article › Research › peer-review

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Histone H4 lysine 20 mono-methylation directly facilitates chromatin openness and promotes transcription of housekeeping genes. / Shoaib, Muhammad; Chen, Qinming; Shi, Xiangyan; Nair, Nidhi; Prasanna, Chinmayi; Yang, Renliang; Walter, David; Frederiksen, Klaus S.; Einarsson, Hjorleifur; Svensson, J. Peter; Liu, Chuan Fa; Ekwall, Karl; Lerdrup, Mads; Nordenskiöld, Lars; Sørensen, Claus S.

In: Nature Communications, Vol. 12, No. 1, 4800, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Shoaib, M, Chen, Q, Shi, X, Nair, N, Prasanna, C, Yang, R, Walter, D, Frederiksen, KS, Einarsson, H, Svensson, JP, Liu, CF, Ekwall, K, Lerdrup, M, Nordenskiöld, L & Sørensen, CS 2021, 'Histone H4 lysine 20 mono-methylation directly facilitates chromatin openness and promotes transcription of housekeeping genes', Nature Communications, vol. 12, no. 1, 4800. https://doi.org/10.1038/s41467-021-25051-2

APA

Shoaib, M., Chen, Q., Shi, X., Nair, N., Prasanna, C., Yang, R., Walter, D., Frederiksen, K. S., Einarsson, H., Svensson, J. P., Liu, C. F., Ekwall, K., Lerdrup, M., Nordenskiöld, L., & Sørensen, C. S. (2021). Histone H4 lysine 20 mono-methylation directly facilitates chromatin openness and promotes transcription of housekeeping genes. Nature Communications, 12(1), [4800]. https://doi.org/10.1038/s41467-021-25051-2

Vancouver

Shoaib M, Chen Q, Shi X, Nair N, Prasanna C, Yang R et al. Histone H4 lysine 20 mono-methylation directly facilitates chromatin openness and promotes transcription of housekeeping genes. Nature Communications. 2021;12(1). 4800. https://doi.org/10.1038/s41467-021-25051-2

Author

Shoaib, Muhammad ; Chen, Qinming ; Shi, Xiangyan ; Nair, Nidhi ; Prasanna, Chinmayi ; Yang, Renliang ; Walter, David ; Frederiksen, Klaus S. ; Einarsson, Hjorleifur ; Svensson, J. Peter ; Liu, Chuan Fa ; Ekwall, Karl ; Lerdrup, Mads ; Nordenskiöld, Lars ; Sørensen, Claus S. / Histone H4 lysine 20 mono-methylation directly facilitates chromatin openness and promotes transcription of housekeeping genes. In: Nature Communications. 2021 ; Vol. 12, No. 1.

Bibtex

@article{be0eb2ee12814aab9352f01b9ab0a111,

title = "Histone H4 lysine 20 mono-methylation directly facilitates chromatin openness and promotes transcription of housekeeping genes",

abstract = "Histone lysine methylations have primarily been linked to selective recruitment of reader or effector proteins that subsequently modify chromatin regions and mediate genome functions. Here, we describe a divergent role for histone H4 lysine 20 mono-methylation (H4K20me1) and demonstrate that it directly facilitates chromatin openness and accessibility by disrupting chromatin folding. Thus, accumulation of H4K20me1 demarcates highly accessible chromatin at genes, and this is maintained throughout the cell cycle. In vitro, H4K20me1-containing nucleosomal arrays with nucleosome repeat lengths (NRL) of 187 and 197 are less compact than unmethylated (H4K20me0) or trimethylated (H4K20me3) arrays. Concordantly, and in contrast to trimethylated and unmethylated tails, solid-state NMR data shows that H4K20 mono-methylation changes the H4 conformational state and leads to more dynamic histone H4-tails. Notably, the increased chromatin accessibility mediated by H4K20me1 facilitates gene expression, particularly of housekeeping genes. Altogether, we show how the methylation state of a single histone H4 residue operates as a focal point in chromatin structure control. While H4K20me1 directly promotes chromatin openness at highly transcribed genes, it also serves as a stepping-stone for H4K20me3-dependent chromatin compaction.",

author = "Muhammad Shoaib and Qinming Chen and Xiangyan Shi and Nidhi Nair and Chinmayi Prasanna and Renliang Yang and David Walter and Frederiksen, {Klaus S.} and Hjorleifur Einarsson and Svensson, {J. Peter} and Liu, {Chuan Fa} and Karl Ekwall and Mads Lerdrup and Lars Nordenski{\"o}ld and S{\o}rensen, {Claus S.}",

note = "Publisher Copyright: {\textcopyright} 2021, The Author(s).",

year = "2021",

doi = "10.1038/s41467-021-25051-2",

language = "English",

volume = "12",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "nature publishing group",

number = "1",

}

RIS

TY - JOUR

T1 - Histone H4 lysine 20 mono-methylation directly facilitates chromatin openness and promotes transcription of housekeeping genes

AU - Shoaib, Muhammad

AU - Chen, Qinming

AU - Shi, Xiangyan

AU - Nair, Nidhi

AU - Prasanna, Chinmayi

AU - Yang, Renliang

AU - Walter, David

AU - Frederiksen, Klaus S.

AU - Einarsson, Hjorleifur

AU - Svensson, J. Peter

AU - Liu, Chuan Fa

AU - Ekwall, Karl

AU - Lerdrup, Mads

AU - Nordenskiöld, Lars

AU - Sørensen, Claus S.

PY - 2021

Y1 - 2021

N2 - Histone lysine methylations have primarily been linked to selective recruitment of reader or effector proteins that subsequently modify chromatin regions and mediate genome functions. Here, we describe a divergent role for histone H4 lysine 20 mono-methylation (H4K20me1) and demonstrate that it directly facilitates chromatin openness and accessibility by disrupting chromatin folding. Thus, accumulation of H4K20me1 demarcates highly accessible chromatin at genes, and this is maintained throughout the cell cycle. In vitro, H4K20me1-containing nucleosomal arrays with nucleosome repeat lengths (NRL) of 187 and 197 are less compact than unmethylated (H4K20me0) or trimethylated (H4K20me3) arrays. Concordantly, and in contrast to trimethylated and unmethylated tails, solid-state NMR data shows that H4K20 mono-methylation changes the H4 conformational state and leads to more dynamic histone H4-tails. Notably, the increased chromatin accessibility mediated by H4K20me1 facilitates gene expression, particularly of housekeeping genes. Altogether, we show how the methylation state of a single histone H4 residue operates as a focal point in chromatin structure control. While H4K20me1 directly promotes chromatin openness at highly transcribed genes, it also serves as a stepping-stone for H4K20me3-dependent chromatin compaction.

AB - Histone lysine methylations have primarily been linked to selective recruitment of reader or effector proteins that subsequently modify chromatin regions and mediate genome functions. Here, we describe a divergent role for histone H4 lysine 20 mono-methylation (H4K20me1) and demonstrate that it directly facilitates chromatin openness and accessibility by disrupting chromatin folding. Thus, accumulation of H4K20me1 demarcates highly accessible chromatin at genes, and this is maintained throughout the cell cycle. In vitro, H4K20me1-containing nucleosomal arrays with nucleosome repeat lengths (NRL) of 187 and 197 are less compact than unmethylated (H4K20me0) or trimethylated (H4K20me3) arrays. Concordantly, and in contrast to trimethylated and unmethylated tails, solid-state NMR data shows that H4K20 mono-methylation changes the H4 conformational state and leads to more dynamic histone H4-tails. Notably, the increased chromatin accessibility mediated by H4K20me1 facilitates gene expression, particularly of housekeeping genes. Altogether, we show how the methylation state of a single histone H4 residue operates as a focal point in chromatin structure control. While H4K20me1 directly promotes chromatin openness at highly transcribed genes, it also serves as a stepping-stone for H4K20me3-dependent chromatin compaction.

U2 - 10.1038/s41467-021-25051-2

DO - 10.1038/s41467-021-25051-2

M3 - Journal article

C2 - 34417450

AN - SCOPUS:85113228833

VL - 12

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 4800

ER -

ID: 277235646