Functional characterisation of human glycine receptors in a fluorescence-based high throughput screening assay
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Functional characterisation of human glycine receptors in a fluorescence-based high throughput screening assay. / Jensen, Anders A.
In: European Journal of Pharmacology, Vol. 521, No. 1-3, 2005, p. 39-42.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Functional characterisation of human glycine receptors in a fluorescence-based high throughput screening assay
AU - Jensen, Anders A.
PY - 2005
Y1 - 2005
N2 - The human glycine receptor subtypes alpha1beta and alpha2 have been expressed stably in HEK293 cells, and the functional characteristics of the receptors have been characterised in the FLIPR Membrane Potential Assay. The pharmacological properties obtained for nine standard ligands at the two receptors in this assay were found to be in good agreement with those from electrophysiology studies of the receptors expressed in Xenopus oocytes or mammalian cell lines. Hence, this high throughput screening assay will be of great use in future pharmacological studies of glycine receptors, particular in the search for novel compound structures acting at them.
AB - The human glycine receptor subtypes alpha1beta and alpha2 have been expressed stably in HEK293 cells, and the functional characteristics of the receptors have been characterised in the FLIPR Membrane Potential Assay. The pharmacological properties obtained for nine standard ligands at the two receptors in this assay were found to be in good agreement with those from electrophysiology studies of the receptors expressed in Xenopus oocytes or mammalian cell lines. Hence, this high throughput screening assay will be of great use in future pharmacological studies of glycine receptors, particular in the search for novel compound structures acting at them.
KW - Androstanes
KW - Azasteroids
KW - Cell Line
KW - Dose-Response Relationship, Drug
KW - Fluorescence
KW - Glycine
KW - Glycine Agents
KW - Humans
KW - Membrane Potentials
KW - Patch-Clamp Techniques
KW - Picrotoxin
KW - Receptors, Glycine
KW - Strychnine
KW - Taurine
KW - Transfection
KW - beta-Alanine
U2 - 10.1016/j.ejphar.2005.08.002
DO - 10.1016/j.ejphar.2005.08.002
M3 - Journal article
C2 - 16182281
VL - 521
SP - 39
EP - 42
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 1-3
ER -
ID: 38484833